EGR1 Antibody

Western Blot: EGR1 Antibody [NBP1-02807] - Affinity Purified anti-EGR-1 antibody shows detection of a predominant band at ~58 kD corresponding to EGR-1 present in mouse embryonic fibroblast whole cell lysate (arrowhead). Approximately 35 ug of lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1500. Reaction occurred 2 hours at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (for 45 min at room temperature.

Immunohistochemistry-Paraffin: EGR1 Antibody [NBP1-02807] - Staining of human lung small cell carcinoma tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Product Discontinued

EGR1 Antibody Summary

• Immunogen: Synthetic peptide corresponding to an internal region near aa 85-115 of Human EGR1.
• Predicted Species: Chimpanzee (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EGR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:4000 - 1:16000
  • Immunohistochemistry 10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 1:500 - 1:3000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.2) and 0.15M NaCl
• Preservative: 0.01% Sodium Azide
• Concentration: 0.93 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for EGR1 Antibody

• AT225Transcription factor ETR103
• early growth response 1
• early growth response protein 1
• EGR1
• EGR-1
• G0S30
• KROX24
• KROX-24
• Nerve growth factor-induced protein A
• NGFIA
• NGFI-ATranscription factor Zif268
• TIS8
• ZIF-268
• Zinc finger protein Krox-24
• ZNF225
• ZNF225Zinc finger protein 225

Background

EGR-1 (also called Early Growth Response protein 1, Krox-24 protein, ZIF268, Nerve growth factor-induced protein A or NGFI-A, Transcription factor ETR103, and Zinc finger protein 225 or AT225) is a transcriptional regulator that recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3' (EGR-site). EGR-1 activates the transcription of target genes whose products are required for mitogenesis and differentiation. EGR-1 is a nuclear protein induced by growth factors. Expression has been identified in a variety of cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EGR1
• Entrez:
  • 1958(Human)
• Uniprot:
  • P18146()