EGLN3/PHD3 Antibody [Alexa Fluor™ Plus 594]

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, ChIP, ICC/IF, IHC, IP, WB, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Alexa Fluor Plus 594

EGLN3/PHD3 Antibody [Alexa Fluor™ Plus 594] Summary

• Immunogen: A synthetic peptide made to the C-terminus of humanPHD3/HIF Prolyl Hydroxylase 3. [LocusLink ID 112399]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EGLN3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Chromatin Immunoprecipitation
  • Immunoblotting
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Primate reactivity reported in scientific literature (PMID: 23732909). Rat reactivity reported in scientific literature (PMID: 23545307).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for EGLN3/PHD3 Antibody [Alexa Fluor™ Plus 594]

• EC 1.14.11
• EC 1.14.11.-
• EGL nine (C.elegans) homolog 3
• egl nine homolog 3 (C. elegans)
• egl nine homolog 3
• egl nine-like protein 3 isoform
• EGLN3
• FLJ21620
• HIF prolyl hydroxylase 3
• HIFPH3 MGC125998
• HIFPH3
• HIF-PH3
• HIF-prolyl hydroxylase 3
• HPH-1
• HPH-3
• Hypoxia-inducible factor prolyl hydroxylase 3
• MGC125999
• pdh3
• PHD3
• Prolyl hydroxylase domain-containing protein 3

Background

HIF prolyl hydroxylase 3 (HPH-3; PHD3; EGLN3) catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins, hydroxylates HIF-1 alpha at Pro-564, and also hydroxylates HIF-2 alpha. PHD3 functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex. EGLN3 may play a role in cell growth regulation in muscle cells and in apoptosis in neuronal tissue. HIF prolyl hydroxylase 3 promotes cell death through a caspase-dependent mechanism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EGLN3