Recombinant Human EGF Protein

Images

 
Functional: Human EGF Protein [NBP2-34952]
SDS-Page: Human EGF Protein [NBP2-34952]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func, PAGE
Format
Carrier-Free
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Human EGF Protein Summary

Description
A biologically active protein corresponding to 53 residues of EGF.

Source: E. coli

Amino Acid Sequence: NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR
Preparation
Method
Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance.
Details of Functionality
EGF protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 0.1 ng/ml, corresponding to a specific activity of >1.0 x 10^7 IU/mg.
Protein/Peptide Type
Biologically Active Protein
Gene
EGF
Purity
> 98 % pure by SDS-PAGE and HPLC
Endotoxin Note
Less than 1 EU/ug of endotoxin as determined by LAL method.

Applications/Dilutions

Theoretical MW
6.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-34952 in the following application:

Publications
Read Publications using NBP2-34952.

Packaging, Storage & Formulations

Storage
Store at 4C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20C. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Concentration
LYOPH
Purity
> 98 % pure by SDS-PAGE and HPLC
Reconstitution Instructions
Reconstitute with sterilized distilled water or 0.1% BSA aqueous buffer to a final concentration of 0.1 - 1.0 mg/ml.

Notes

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Alternate Names for Recombinant Human EGF Protein

  • beta-urogastrone
  • EGF
  • epidermal growth factor (beta-urogastrone)
  • epidermal growth factor
  • HOMG4
  • pro-epidermal growth factor
  • URG
  • Urogastrone

Background

EGF R (ErbB1) is a receptor tyrosine kinase that is widely expressed on epithelial cells and is required for epithelial cell development and proliferation. EGF R binds the EGF family ligands: EGF, Amphiregulin, TGF-alpha, Betacellulin, Epiregulin, HB-EGF, and Epigen. Ligand binding induces EGF R homodimerization as well as heterodimerization with ErbB2, ErbB3, or ErbB4. EGF R signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis. Alterations in the structure, expression, and signaling of EGF R are involved in the development and metastasis of a wide variety of cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF1095
Species: Hu
Applications: WB, Simple Western, IHC, CyTOF-ready, ICC, ICFlow
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
7270-IL/CF
Species: Hu
NB100-56749
Species: Hu, Mu
Applications: WB, IHC, IHC-P
AF467
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
NBP2-37576
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC
NBP2-50037
Species: Hu, Mu, Rt, Po, Bv, Eq
Applications: WB, ICC/IF
NBP2-34952
Species: Hu
Applications: Func, PAGE

Publications for EGF Protein (NBP2-34952)(11)


Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NBP2-34952 Applications Species
Wucheng Wang, Huanjun Chen, Lei Zhang et al. A fucoidan from Nemacystus decipiens disrupts angiogenesis through targeting bone morphogenetic protein 4. Carbohydr Polym  2016 Jun 25 [PMID: 27083822]
Liu, Wanjun PhD, Wang et al. CYP2J2 Overexpression Increases EETs and Protects Against HFD-Induced Atherosclerosis in ApoE−/− Mice. Journal of Cardiovascular Pharmacology 2016 Jun [PMID: 26859195]
Yan Luan, Dafei Chai, Jianjian Peng et al. A fully human monoclonal antibody targeting PD-L1 with potent anti-tumor activity. Int Immunopharmacol  2016 Feb [PMID: 26773772]
Jiayin Hou, Liang Liu, Qian Zhu et al. MicroRNA‐185 inhibits angiogenesis in human microvascular endothelial cells through targeting stromal interaction molecule 1. Cell Biol Int 2016 Mar [PMID: 26694763]
Yu Ma, Fu-Chun Guo, Wei Wang et al. K-ras gene mutation as a predictor of cancer cell responsiveness to metformin. Mol Med Rep  2013 Sep [PMID: 23877793]
Xia Chen, Fei Xiao, Ying Wang et al. Structure-activity relationship study of WSS25 derivatives with anti-angiogenesis effects. Glycoconj J 2012 Aug [PMID: 22847113]
Yuxia Xu, Qun Dong, Hong Qiu et al. A homogalacturonan from the radix of Platycodon grandiflorum and the anti-angiogenesis activity of poly-/oligogalacturonic acids derived therefrom. Carbohydr Res 2011 Sep 27 [PMID: 21700274]
Xiaokun Shen, Jianping Fang, Xiaofen Lv et al. Heparin Impairs Angiogenesis through Inhibition of MicroRNA-10b. J Biol Chem 2011 Jul 29 [PMID: 21642433]
Yuxia Xu, Qun Dong, Hong Qiu et al. Structural Characterization of an Arabinogalactan from Platycodon grandiflorum Roots and Antiangiogenic Activity of Its Sulfated Derivative. Biomacromolecules  2010 Oct 11 [PMID: 20839877]
Hong Qiu, Bo Yang, Zhi-Chao Pei, et al. WSS25 Inhibits Growth of Xenografted Hepatocellular Cancer Cells in Nude Mice by Disrupting Angiogenesis via Blocking Bone Morphogenetic Protein (BMP)/Smad/Id1 Signaling. J Biol Chem 2010 Oct 15 [PMID: 20679340]
Show All 11 Publications.

Review for EGF Protein (NBP2-34952) (1) 51

Average Rating: 5
(Based on 1 review)

Reviews using NBP2-34952:
Filter by Applications
All Applications
Filter by Species
All Species
Images Ratings Applications Species Date Details
 EGF NBP2-34952
Enlarge
reviewed by:
Anonymous
01/04/2017
View

Summary

CommentsI ranked this protein with 5 stars because the cell differentiation was successfully performed.

Comments

CommentsSDS PAGE gel with Coomassie blue staining showing detection of Human EGF protein (NBP2-34952-100 ug ) (5 ug).

FAQs for EGF Protein (NBP2-34952) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional EGF Products

Bioinformatics Tool for EGF Protein (NBP2-34952)

Discover related pathways, diseases and genes to EGF Protein (NBP2-34952). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for EGF Protein (NBP2-34952)

Discover more about diseases related to EGF Protein (NBP2-34952).
 

Pathways for EGF Protein (NBP2-34952)

View related products by pathway.

PTMs for EGF Protein (NBP2-34952)

Learn more about PTMs related to EGF Protein (NBP2-34952).
 

Research Areas for EGF Protein (NBP2-34952)

Find related products by research area.

Blogs on EGF.

Using EGF Protein from Novus Biologicals
EGF (epidermal growth factor) stimulates differentiation, proliferation and cell growth by binding to its receptor, EGFR. EGF was first discovered in the mouse submandibular gland in 1986 by Stanley Cohen of Vanderbilt University, leading to a Nobel P...  Read full blog post.

Myosin is More than Just a Heavy Lifter
Myosin is a well-known, hexameric molecular motor that is a key cytoskeletal component. It consists of a pair of myosin heavy chain subunits (MHC), a pair of essential myosin light chain subunits (MLC), and a pair of regulatory light chain subunits (R...  Read full blog post.

Customers Who Bought This Also Bought

IL-6 [Unconjugated]
7270-IL-025/CF

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
01/04/2017
Application:
Species:

Bioinformatics

Gene Symbol EGF
Entrez
Uniprot