Recombinant Human EGF Protein Summary
A biologically active protein corresponding to 53 residues of EGF.
Source: E. coli
Amino Acid Sequence: NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR
Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance.
|Details of Functionality
EGF protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 0.1 ng/ml, corresponding to a specific activity of >1.0 x 10^7 IU/mg.
| Protein/Peptide Type
Biologically Active Protein
>95% pure by SDS-PAGE
Less than 1 EU/ug of endotoxin as determined by LAL method.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 1 Review rated 5 using
NBP2-34952 in the following application:
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
>95% pure by SDS-PAGE
Reconstitute with sterilized distilled water or 0.1% BSA aqueous buffer to a final concentration of 0.1 - 1.0 mg/ml.
This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
Alternate Names for Recombinant Human EGF Protein
- epidermal growth factor (beta-urogastrone)
- epidermal growth factor
- pro-epidermal growth factor
EGF R (ErbB1) is a receptor tyrosine kinase that is widely expressed on epithelial cells and is required for epithelial cell development and proliferation. EGF R binds the EGF family ligands: EGF, Amphiregulin, TGF-alpha, Betacellulin, Epiregulin, HB-EGF, and Epigen. Ligand binding induces EGF R homodimerization as well as heterodimerization with ErbB2, ErbB3, or ErbB4. EGF R signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis. Alterations in the structure, expression, and signaling of EGF R are involved in the development and metastasis of a wide variety of cancers.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed
for 2 years from date of receipt.
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Publications for EGF Protein (NBP2-34952) (0)
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Review for EGF Protein (NBP2-34952) (1)
Average Rating: 5
(Based on 1 review)
Reviews using NBP2-34952:
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|Comments||I ranked this protein with 5 stars because the cell differentiation was successfully performed.|
|Comments||SDS PAGE gel with Coomassie blue staining showing detection of Human EGF protein (NBP2-34952-100 ug ) (5 ug).|
FAQs for EGF Protein (NBP2-34952) (0)
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Bioinformatics Tool for EGF Protein (NBP2-34952)
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