EGF Antibody (463004) [Alexa Fluor® 350]

• Applications: WB, IHC
• Clone: 463004
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 350

EGF Antibody (463004) [Alexa Fluor® 350] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse EGF
  Trp29-Arg1029
  Accession # P01132
• Specificity: Detects mouse Pro EGF in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) Amphiregulin, rmEpigen, rmEpiregulin, rmEGF, rmMFG-E8, recombinant human (rh) EGF, rhHB-EGF, rhHRG alpha, rhHRG beta, or recom
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

EGF is the prototypic member of a family of growth factors that also includes amphiregulin, betacellulin, epigen, epiregulin, HB-EGF, neuregulins-1 through -6, and TGF-alpha (1). These proteins contain EGF-like domains with three intramolecular disulfide bonds between conserved cysteines (2). EGF family members are synthesized as transmembrane preproproteins with varying numbers of EGF-like domains (3). The extracellular region of mouse pro-EGF contains eight LDL R class B repeats and nine EGF-like domains (4). Mature EGF is derived from the juxtamembrane EGF-like domain. EGF binds ErbB1 and induces the formation of homodimers or heterodimers containing ErbB2 (5). Pro-EGF is most highly expressed in the submaxillary gland and kidney (6). In the kidney, the 190 kDa preproprotein is cleaved by membrane-associated serine proteases, liberating the extracellular region which is subsequently processed into smaller fragments including the 6 kDa mature EGF (7‑10). The various cleavage products produced in the kidney also are present in urine (9‑11). In the submaxillary gland, however, nearly all EGF is processed intracellularly and stored in secretory vesicles (6, 12). The soluble precursor binds ErbB1 and induces cellular proliferation, although it is significantly less potent than mature EGF (8, 9). In human thyroid carcinoma cells, a splice variant of pro-EGF with a deletion in the cytoplasmic domain induces increased proliferative activity relative to wild-type pro-EGF (13). Within the extracellular region, mouse pro-EGF shares 79% amino acid sequence identity with rat pro-EGF and 67%‑69% with human, canine, feline, and porcine pro-EGF.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.