EFHD1 Antibody

Images

 
Western Blot: EFHD1 Antibody [NBP1-77134] - human spleen tissue lysate with EFHD1 antibody at (A) 2 and (B) 4 ug/ml.
Immunocytochemistry/ Immunofluorescence: EFHD1 Antibody [NBP1-77134] - Rat spleen tissue with EFHD1 antibody at 5 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

EFHD1 Antibody Summary

Immunogen
Antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human EFHD1. The immunogen is located within the first 50 amino acids of EFHD1.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EFHD1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 2-4 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunofluorescence 20 ug/ml
Positive Control
Spleen Lysate (NB820-59259)
Control Peptide
EFHD1 Peptide (NBP1-77134PEP)
Publications
Read Publication using NBP1-77134.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EFHD1 Antibody

  • DKFZp781H0842
  • EF-hand domain family, member D1
  • EF-hand domain-containing protein 1
  • EF-hand domain-containing protein D1
  • member D1
  • MST133
  • MSTP133
  • swiprosin-2

Background

EFHD1, also known as Swiprosin-2 or SWS2, is an EF-hand and coiled-coil-containing adaptor protein identified in a subtractive hybridization study using a neuronal cell line established from the cerebellum of an adult p53-null mouse, however further study indicated no difference in normal mice. Its mRNA is widely expressed, with its expression in brain undetectable at embryonic stages, with increasing levels from postnatal to adult development. In situ hybridization showed expression in neurons but not white matter of the cerebellum and cerebrum. EHFD1 is also highly expressed in testes, ovary, and the collecting ducts of the kidney, suggesting that in non-neuronal cells, EFHD1 may be involved in gametogenesis and water-reabsorbtion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EFHD1 Antibody (NBP1-77134)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for EFHD1 Antibody (NBP1-77134). (Showing 1 - 1 of 1 FAQ).

  1. I have used your EFHD1 antibody (NBP1-71699) and can detect a band at approximately 150kDa. Have you detected this band as well?
    • EFHD1 protein is 239 amino acids in length with a molecular weight of 27kDa. That is the band that our antibody is recognizing. We have not observed the 150kDa band that you are referring to and cannot comment on its origin. You should observe a band at approximately 27kDa on your Western blot.

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Bioinformatics

Gene Symbol EFHD1
Entrez
Uniprot