Human EDNRB/Endothelin R Type B ELISA Kit (Colorimetric)

ELISA: Human EDNRB/Endothelin R Type B ELISA Kit (Colorimetric) [NBP2-80133] - Samples were spiked with high concentrations of Human EDNRB/Endothelin R Type B and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human EDNRB/Endothelin R Type B ELISA Kit (Colorimetric) [NBP2-80133] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, and other biological fluids.
• Standard Curve Range: 0.31-20 ng/mL
• Sensitivity: 0.19 ng/mL

Human EDNRB/Endothelin R Type B ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human ETRB in samples. No Significant cross-reactivity or interference between Human ETRB and analogues was observed.
• Standard Curve Range: 0.31-20 ng/mL
• Sensitivity: 0.19 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.14%
• Intra-Assay: CV% < 4.73%
• Spike Recovery: 91-109%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: EDNRB

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human EDNRB/Endothelin R Type B ELISA Kit (Colorimetric)

• ABCDS
• EDNRB
• endothelin B receptor
• Endothelin receptor non-selective type
• endothelin receptor type B
• ETB
• ET-B
• ETBR
• ET-BR
• ETRB
• HSCR
• HSCR2
• WS4A

Background

The Endothelin Receptor ETB is expressed in most tissues. Mutations of the receptor gene are responsible for Hirschsprung disease type 2, a multigenic disorder with phenotypes such as bicolored irides, megacolon, hypopigmentation, and hearing loss. The ETB receptor has similar affinity for all three endothelins and activates a phosphatidylinositol-calcium second messenger system. A splice variant, termed SVR, has been described; the sequence of the ETB-SVR receptor is identical to ET-B receptor, with the exception of the intracellular C-terminal domain. While both splice variants bind endothelin ET1, they exhibit different responses, which suggests that they may be functionally distinct. The endothelin B receptor has been reported to be expressed in adrenal, aorta, artery, brain, breast, eye, epididymis, ganglion, heart, kidney, liver, lung, ovary, placenta, prostate, skin, testis, uterus, and vessel. ESTs have been isolated from amnion, B-cell/lung/testis, breast, brain, colon, ear, embryo, eye, heart, kidney, liver, liver/spleen, lung, lymph node, placenta, prostate, and skin libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for EDNRB/Endothelin R Type B ELISA Kit (NBP2-80133). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: EDNRB
• Uniprot:
  • P24530()
  • P24530()