EDNRB/Endothelin R Type B Antibody

Western Blot: EDNRB/Endothelin R Type B Antibody [NBP1-31108] - A. A375 whole cell lysate/extract

Western Blot: EDNRB/Endothelin R Type B Antibody [NBP1-31108] - Rat 2 whole cell lysate/extract.

Product Discontinued

EDNRB/Endothelin R Type B Antibody Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human EDNRB/Endothelin R Type B. The exact sequence is proprietary.
• Localization: Cell membrane
• Predicted Species: Mouse (100%), Porcine (100%), Rabbit (100%), Canine (100%), Chicken (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EDNRB
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Application Notes: IHC-P reactivity reported in (PMID: 26980806). ICC/IF reactivity reported in (PMID: 25370848).
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Bovine reactivity reported in (PMID:26980806), Equine reactivity reported in (PMID: 25370848).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for EDNRB/Endothelin R Type B Antibody

• ABCDS
• EDNRB
• endothelin B receptor
• Endothelin receptor non-selective type
• endothelin receptor type B
• ETB
• ET-B
• ETBR
• ET-BR
• ETRB
• HSCR
• HSCR2
• WS4A

Background

The Endothelin Receptor ETB is expressed in most tissues. Mutations of the receptor gene are responsible for Hirschsprung disease type 2, a multigenic disorder with phenotypes such as bicolored irides, megacolon, hypopigmentation, and hearing loss. The ETB receptor has similar affinity for all three endothelins and activates a phosphatidylinositol-calcium second messenger system. A splice variant, termed SVR, has been described; the sequence of the ETB-SVR receptor is identical to ET-B receptor, with the exception of the intracellular C-terminal domain. While both splice variants bind endothelin ET1, they exhibit different responses, which suggests that they may be functionally distinct. The endothelin B receptor has been reported to be expressed in adrenal, aorta, artery, brain, breast, eye, epididymis, ganglion, heart, kidney, liver, lung, ovary, placenta, prostate, skin, testis, uterus, and vessel. ESTs have been isolated from amnion, B-cell/lung/testis, breast, brain, colon, ear, embryo, eye, heart, kidney, liver, liver/spleen, lung, lymph node, placenta, prostate, and skin libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for EDNRB/Endothelin R Type B Antibody (NBP1-31108)(2)

Publications using NBP1-31108

• Kobayashi Y, Yoshimoto Y, Yamamoto Y et al. Roles of EDNs in regulating oviductal NO synthesis and smooth muscle motility in cows. Reproduction. 2016-03-15 [PMID: 26980806] (IHC-P, Bovine)
• Yamamoto, Y, Kohka, M Et al. Endothelin as a local regulating factor in the bovine oviduct. Reprod Fertil Dev 2016-04-01 [PMID: 25370848] (ICC/IF, Equine)
  
  Details:
  Citation using the IHC-Prediluted format of this antibody.

Bioinformatics

• Gene Symbol: EDNRB
• Entrez:
  • 1910(Human)
• Uniprot:
  • P24530()