EDNRB/Endothelin R Type B Antibody (671917) [Unconjugated]

Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human EDNRB/Endothelin R Type B. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human EDNRB/Endothelin R Type B Monoclonal Antibody (Catalog # MAB4496) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for EDNRB/Endothelin R Type B at approximately 77 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

EDNRB/Endothelin R Type B was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human EDNRB/Endothelin R Type B Monoclonal Antibody (Catalog # MAB4496) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to smooth muscle. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

HEK293 human embryonic kidney cell line transfected with (A) human EDNRB or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human EDNRB Monoclonal Antibody (Catalog # MAB4496) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on Mouse IgG2A Isotype Control (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: WB, Flow, IHC
• Clone: 671917
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

EDNRB/Endothelin R Type B Antibody (671917) [Unconjugated] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human EDNRB/Endothelin R Type B
  Accession # P24530
• Specificity: Detects human EDNRB/Endothelin R Type B in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: EDNRB

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 1-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EDNRB/Endothelin R Type B Antibody (671917) [Unconjugated]

• ABCDS
• EDNRB
• endothelin B receptor
• Endothelin receptor non-selective type
• endothelin receptor type B
• ETB
• ET-B
• ETBR
• ET-BR
• ETRB
• HSCR
• HSCR2
• WS4A

Background

EDNRB (Endothelin Receptor Type B) is a 50-55 kDa member of the beta-family of rhodopsin receptors. It binds endothelin 1, 2 and 3, and is found on endothelial cells where it mediates vasodilation. Mature human EDNRB is a 7-transmembrane glycoprotein that is 416 amino acids (aa) in length. It contains a 75 aa N-terminal extracellular region (aa 27-101), and a 44 aa C-terminal cytoplasmic domain. There are three EDNRB variants that affect aa 27-101. One shows a 90 aa N-terminal extension, a second shows the same 90 aa N-terminal substitution coupled with a deletion of aa 268-398, and a third shows proteolytic cleavage between Arg64‑Ser65. Over aa 27-101, human EDNRB shares 67% and 97% aa identity with mouse and canine EDNRB, respectively.     

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EDNRB
• Uniprot:
  • P24530()