EDF1 Antibody - BSA Free

Western Blot: EDF1 Antibody [NBP2-92882] - Analysis of extracts of various cell lines, using EDF1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.

Immunohistochemistry-Paraffin: EDF1 Antibody [NBP2-92882] - Rat brain using EDF1 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry-Paraffin: EDF1 Antibody [NBP2-92882] - Human gastric cancer using EDF1 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry-Paraffin: EDF1 Antibody [NBP2-92882] - Mouse lung using EDF1 antibody at dilution of 1:100 (40x lens).

Western Blot: EDF1 Antibody [NBP2-92882] - Analysis of extracts from normal (control) and EDF1 Rabbit pAb knockout (KO) NIH/3T3 cells, using EDF1 Rabbit pAb antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

EDF1 Antibody - BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-148 of human EDF1 (NP_003783.1). MAESDWDTVTVLRKKGPTAAQAKSKQAILAAQRRGEDVETSKKWAAGQNKQHSITKNTAKLDRETEELHHDRVTLEVGKVIQQGRQSKGLTQKDLATKINEKPQVIADYESGRAIPNNQVLGKIERAIGLKLRGKDIGKPIEKGPRAK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EDF1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin
  • Knockout Validated
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for EDF1 Antibody - BSA Free

• EDF-1Multiprotein-bridging factor 1
• endothelial differentiation-related factor 1
• MBF1
• MGC9058
• multiprotein bridging factor 1
• multiprotein bridging factor-1

Background

EDF1 encodes a protein that may regulate endothelial cell differentiation. It has been postulated that the protein functions as a bridging molecule that interconnects regulatory proteins and the basal transcriptional machinery, thereby modulating the transcription of genes involved in endothelial differentiation. This protein has also been found to act as a transcriptional coactivator by interconnecting the general transcription factor TATA element-binding protein (TBP) and gene-specific activators. Two alternatively spliced transcripts which encode distinct proteins have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EDF1