EBP Antibody - BSA Free

Western Blot: EBP Antibody [NBP3-06227] - Anti-EBP rabbit polyclonal antibody at 1:500 dilutionLane A: MCF-7 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: A549 Whole Cell LysateLane D: 293T Whole Cell LysateLysatesroteins at 30 ug per lane.Secondary: Goat Anti-Rabbit IgG (H+L)HRP at 110000 dilution.Developed using the ECL technique. Performed under reducing conditions.Predicted band size:26 kDaObserved band size:28 kDa(We are unsure as to the identity of these extra bands.)

Immunocytochemistry/Immunofluorescence: EBP Antibody [NBP3-06227] - Immunofluorescence staining of EBP in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human EBP polyclonal antibody (dilution ratio 1:200) at 4 degrees celsius overnight. Then cells were stained with the Alexa Fluor (TM) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

Immunohistochemistry-Paraffin: EBP Antibody [NBP3-06227] - Immunochemical staining of human EBP in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

EBP Antibody - BSA Free Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human EBP fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EBP
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for EBP Antibody - BSA Free

• 3-beta-hydroxysteroid-Delta(8)
• CDPX2
• Cholestenol Delta-isomerase
• CPX3-beta-hydroxysteroid-delta-8
• CPXDX-linked dominant (Happle syndrome)
• D8-D7 sterol isomerase
• Delta(7)-isomerase
• Delta(8)-Delta(7) sterol isomerase
• delta-7-isomerase
• EBP
• EC 5.3.3.5
• emopamil binding protein (sterol isomerase)
• Emopamil-binding protein
• sterol 8-isomerase

Background

The protein encoded by the EBP gene is an integral membrane protein of the endoplasmic reticulum. It is a high affinity binding protein for the antiischemic phenylalkylamine Ca2+ antagonist (3H)emopamil and the photoaffinity label (3H)azidopamil. It is similar to sigma receptors and may be a member of a superfamily of high affinity drug-binding proteins in the endoplasmic reticulum of different tissues. This protein shares structural features with bacterial and eukaryontic drug transporting proteins. It has four putative transmembrane segments and contains two conserved glutamate residues which may be involved in the transport of cationic amphiphilics. Another prominent feature of this protein is its high content of aromatic amino acid residues (>23%) in its transmembrane segments. These aromatic amino acid residues have been suggested to be involved in the drug transport by the P-glycoprotein. Mutations in this gene cause Chondrodysplasia punctata 2 (CDPX2; also known as Conradi-Hunermann syndrome). (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EBP