![Western Blot: EBF3 Antibody [NBP2-16254] - Sample (30 ug of whole cell lysate) A: U87-MG 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/EBF3-Antibody-Western-Blot-NBP2-16254-img0001.jpg "Western Blot: EBF3 Antibody [NBP2-16254] - Sample (30 ug of whole cell lysate) A: U87-MG 7. 5% SDS PAGE gel, diluted at 1:1000.")
| Reactivity | Hu, Mu, Rt, XpSpecies Glossary |
| Applications | WB, ICC/IF, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | Novus Biologicals Rabbit EBF-3 Antibody - BSA Free (NBP2-16254) is a polyclonal antibody validated for use in WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human EBF-3. The exact sequence is proprietary. |
| Localization | Nucleus |
| Predicted Species | Mouse (99%), Rat (99%), Xenopus (95%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | EBF3 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 65 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: EBF-3 Antibody [NBP2-16254] - U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EBF3 protein stained by EBF3 antibody [C1C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um](http://images.novusbio.com/fullsize/EBF-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16254-img0003.jpg "Immunocytochemistry/Immunofluorescence: EBF-3 Antibody [NBP2-16254] - U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EBF3 protein stained by EBF3 antibody [C1C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um")
![Immunoprecipitation: EBF-3 Antibody [NBP2-16254] - Immunoprecipitation of EBF3 protein from U87-MG whole cell extracts using 5 ug of EBF3 antibody [C1C3] Western blot analysis was performed using EBF3 antibody [C1C3] Easy Blot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/EBF-3-Antibody-Immunoprecipitation-NBP2-16254-img0006.jpg "Immunoprecipitation: EBF-3 Antibody [NBP2-16254] - Immunoprecipitation of EBF3 protein from U87-MG whole cell extracts using 5 ug of EBF3 antibody [C1C3] Western blot analysis was performed using EBF3 antibody [C1C3] Easy Blot anti-Rabbit IgG was used as a secondary reagent.")
![Western Blot EBF-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/EBF-1_AF5165_Western_Blot_5645.jpg)
![Western Blot EBF-1 Antibody [Unconjugated]](https://images.novusbio.com/images/af5165_human-mouse-ebf-1-affinity-purified-polyclonal-ab-41202412403559.jpg)
![Western Blot EBF-1 Antibody [Unconjugated]](https://images.novusbio.com/images/af5165_human-mouse-ebf-1-affinity-purified-polyclonal-ab-41202412403592.jpg)
![Western Blot EBF-2 Antibody [Unconjugated]](https://images.novusbio.com/images2/EBF-2_AF7006_Western_Blot_11279.jpg)
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")