EBF-2 Antibody [Unconjugated]

Western blot shows lysates of 3T3-L1 mouse embryonic fibroblast adipose-like cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse EBF-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7006) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for EBF-2 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

EBF-2 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Sheep Anti-Mouse EBF-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7006) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to developing muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

EBF-2 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse EBF-2
  Arg407-Ser519
  Accession # O08792
• Specificity: Detects mouse EBF-2 in direct ELISAs. Detects mouse and human EBF-2 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: EBF2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EBF-2 Antibody [Unconjugated]

• COE2
• EBF2
• EBF-2
• Mmot1
• O/E-3
• OE-3

Background

EBF-2 (Early B cell Factor 2; also Mmot1, OLF3 and COE2) is a 62 kDa (predicted) member of the COE family of transcription factors. It is expressed in immature osteoblasts and Purkinje cells, and in the embryo is associated with the migration of postmitotic neuroblasts. In immature osteoblasts, EBF-2 appears to upregulate OPG, suppressing osteoclast formation. And in the developing retina, EBF-2 is found in ganglion, glycinergic Amacrine and horizontal cells, possibly promoting their development over that of photoreceptor cells. Mouse EBF-2 is 575 amino acids (aa) in length. It contains one DNA-binding region with an embedded C5-type zinc‑finger motif (aa 62-238), a dimerization ITP/TIG domain (aa 253-336), and a Pro/Ser-rich transactivation domain (aa 453-534). Although considered an HLH type transcription factor, it does not contain the typical "b", or basic amino acid sequence associated with bHLH factors. EBF-2 both homodimerizes, and heterodimerizes with EBF-1 and -3. There is an alternative start site at Met23. Over aa 407-519, mouse EBF-2 is identical in aa sequence to rat EBF-2 and shares 99% aa identity with human EBF-2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EBF-2 Antibody (AF7006)(15)

Publications using AF7006

• ES Liau, S Jin, YC Chen, WS Liu, M Calon, S Nedelec, Q Nie, JA Chen Single-cell transcriptomic analysis reveals diversity within mammalian spinal motor neurons Oncogene, 2023-01-03;14(1):46. 1/3/2023 [PMID: 36596814]
• Cheuk Wang Fung, Shaopu Zhou, Han Zhu, Xiuqing Wei, Zhenguo Wu, Angela Ruohao Wu Cell fate determining molecular switches and signaling pathways in Pax7-expressing somitic mesoderm Cell Discovery 6/28/2022 [PMID: 35764624]
• Margo P. Emont, Christopher Jacobs, Adam L. Essene, Deepti Pant, Danielle Tenen, Georgia Colleluori et al. A single-cell atlas of human and mouse white adipose tissue Nature 3/31/2022 [PMID: 35296864]
• KM Choi, JH Kim, X Kong, M Isik, J Zhang, HW Lim, JC Yoon Defective brown adipose tissue thermogenesis and impaired glucose metabolism in mice lacking Letmd1 Cell Reports, 2021-12-14;37(11):110104. 2021-12-14 [PMID: 34910916] (Western Blot, Mouse, Transgenic Mouse)
• S Su, AR Guntur, DC Nguyen, SS Fakory, CC Doucette, C Leech, H Lotana, M Kelley, J Kohli, J Martino, S Sims-Lucas, L Liaw, C Vary, CJ Rosen, AC Brown A Renewable Source of Human Beige Adipocytes for Development of Therapies to Treat Metabolic Syndrome Cell Rep, 2018-12-11;25(11):3215-3228.e9. 2018-12-11 [PMID: 30540952] (ICC, Human)
• S Elattar, M Dimri, A Satyanaray The tumor secretory factor ZAG promotes white adipose tissue browning and energy wasting FASEB J., 2018-03-23;0(0):fj201701465RR. 2018-03-23 [PMID: 29570397] (Western Blot, Mouse)
• Yuta Hiraike, Hironori Waki, Jing Yu, Masahiro Nakamura, Kana Miyake, Gaku Nagano et al. NFIA co-localizes with PPAR gamma and transcriptionally controls the brown fat gene program Nature Cell Biology 9/1/2017 [PMID: 28812581]
• Yuta Hiraike, Hironori Waki, Jing Yu, Masahiro Nakamura, Kana Miyake, Gaku Nagano et al. NFIA co-localizes with PPAR gamma and transcriptionally controls the brown fat gene program Nature Cell Biology 2017-09-01 [PMID: 28812581] (Chromatin Immunoprecipitation (ChIP), Mouse)
• Suzanne N. Shapira, Hee-Woong Lim, Sona Rajakumari, Alexander P. Sakers, Jeff Ishibashi, Matthew J. Harms et al. EBF2 transcriptionally regulates brown adipogenesis via the histone reader DPF3 and the BAF chromatin remodeling complex Genes & Development 4/1/2017 [PMID: 28428261]
• Binbin Lai, Ji-Eun Lee, Younghoon Jang, Lifeng Wang, Weiqun Peng, Kai Ge MLL3/MLL4 are required for CBP/p300 binding on enhancers and super-enhancer formation in brown adipogenesis Nucleic Acids Research 6/20/2017 [PMID: 28398509]
• M Patil, BK Sharma, S Elattar, J Chang, S Kapil, J Yuan, A Satyanaray Id1 Promotes Obesity by Suppressing Brown Adipose Thermogenesis and White Adipose Browning Diabetes, 2017-03-07;0(0):. 2017-03-07 [PMID: 28270523] (Western Blot, Mouse)
• Silvia Velasco A Multi-step Transcriptional and Chromatin State Cascade Underlies Motor Neuron Programming from Embryonic Stem Cells Cell Stem Cell, 2016-12-08;0(0):. 2016-12-08 [PMID: 27939218] (ICC, Immunoprecipitation, Human)
• Rachel R. Stine, Suzanne N. Shapira, Hee-Woong Lim, Jeff Ishibashi, Matthew Harms, Kyoung-Jae Won et al. EBF2 promotes the recruitment of beige adipocytes in white adipose tissue Molecular Metabolism 1/1/2016 [PMID: 26844207]
• Rachel R. Stine, Suzanne N. Shapira, Hee-Woong Lim, Jeff Ishibashi, Matthew Harms, Kyoung-Jae Won et al. EBF2 promotes the recruitment of beige adipocytes in white adipose tissue Molecular Metabolism 2016-01-01 [PMID: 26844207] (Immunohistochemistry, Western Blot, Mouse)
• Zhao X, Li S, Wang G, Yu Q, Lin J A long noncoding RNA transcriptional regulatory circuit drives thermogenic adipocyte differentiation. Mol Cell, 2014-07-04;55(3):372-82. 2014-07-04 [PMID: 25002143] (ChIP, Human)

Bioinformatics

• Gene Symbol: EBF2
• Uniprot:
  • O08792(Mouse)