E6AP/UBE3A Antibody (PCRP-UBE3A-1A2)

Immunocytochemistry/Immunofluorescence: E6AP/UBE3A Antibody (PCRP-UBE3A-1A2) [NBP3-07170] - Immunofluorescent Analysis of PFA-fixed HeLa cells. E6AP/UBE3A Mouse Monoclonal Antibody (PCRP-UBE3A-1A2) followed by IgG-CF488 (green), counterstained with phalloidin.

Flow Cytometry: E6AP/UBE3A Antibody (PCRP-UBE3A-1A2) [NBP3-07170] - Flow Cytometric Analysis of PFA-fixed HeLa cells. E6AP/UBE3A Mouse Monoclonal Antibody (PCRP-UBE3A-1A2) followed by goat anti- Mouse IgG-CF488 (blue); unstained cells (red).

Protein Array: E6AP/UBE3A Antibody (PCRP-UBE3A-1A2) [NBP3-07170] - Analysis of Protein Array containing more than 19,000 full-length human proteins using E6AP/UBE3A Mouse Monoclonal Antibody (PCRP-UBE3A-1A2).

• Reactivity: Hu
• Applications: Flow, ICC/IF, MA
• Clone: PCRP-UBE3A-1A2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

E6AP/UBE3A Antibody (PCRP-UBE3A-1A2) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08913)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Immunogen: Recombinant full-length human E6AP/UBE3A protein (Uniprot: Q05086)
• Localization: Nucleus, Cytoplasm
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: UBE3A
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Protein Array
• Application Notes: Optimal dilution for a specific application should be determined.
• Theoretical MW: 100 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for E6AP/UBE3A Antibody (PCRP-UBE3A-1A2)

• ANCR
• ANCREPVE6AP
• AS
• CTCL tumor antigen se37-2
• E6AP ubiquitin-protein ligase
• E6AP
• E6-AP
• EC 6.3.2.-
• EPVE6AP
• FLJ26981
• HPVE6A
• human papilloma virus E6-associated protein
• Human papillomavirus E6-associated protein
• Oncogenic protein-associated protein E6-AP
• Renal carcinoma antigen NY-REN-54
• UBE3A
• ubiquitin protein ligase E3A
• ubiquitin-protein ligase E3A

Background

UBE3A encodes an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UBE3A
• Uniprot:
  • Q05086(Human)