E2F-1 Antibody (S03-5G3)

Immunocytochemistry/ Immunofluorescence: E2F-1 Antibody (S03-5G3) [NBP3-19622] - Immunofluorescence of E2F-1(green) in Hela cells using NBP3-19622 at dilution 1/50, and DAPI(blue)

Western Blot: E2F-1 Antibody (S03-5G3) [NBP3-19622] - Western blot detection of E2F-1 in C6, 3T3 cell lysates using NBP3-19622. Predicted band size: 47kDa. Observed band size: 70kDa.

Immunohistochemistry-Paraffin: E2F-1 Antibody (S03-5G3) [NBP3-19622] - Immunohistochemistry of E2F-1 in paraffin-embedded Human tonsil using NBP3-19622 at dilution 1/2

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clone: S03-5G3
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

E2F-1 Antibody (S03-5G3) Summary

• Description: Novus Biologicals Rabbit E2F-1 Antibody (S03-5G3) (NBP3-19622) is a recombinant monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human E2F-1 (Uniprot # Q01094)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: E2F1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.0763888888888889
  • Immunocytochemistry/ Immunofluorescence 1/50
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 0.0763888888888889
  • Western Blot 1:500-1:2000
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for E2F-1 Antibody (S03-5G3)

• E2F transcription factor 1
• E2F1
• E2F-1
• PBR3
• PRB-binding protein E2F-1
• RBAP1
• RBAP-1
• RBBP3
• RBBP-3
• RBP3
• Retinoblastoma-associated protein 1
• Retinoblastoma-binding protein 3
• transcription factor E2F1

Background

E2F1 is encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: E2F1