E-Selectin/CD62E Antibody

Images

 
Western Blot: E-Selectin/CD62E Antibody [NBP1-45545] - Jurkat cell lysate (80 ug) loaded Blocking/Dilution buffer: 5% NFDM (1 hr at RT) / TBST E-Selectin Polyclonal Antibody used at 1:1000 dilution - O/N at 4 degrees C. ...read more
Immunocytochemistry/ Immunofluorescence: E-Selectin/CD62E Antibody [NBP1-45545] - Analysis of E-Selectin in formaldehyde fixed, porcine cardiac tissue using anti-E-Selectin antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: E-Selectin/CD62E Antibody [NBP1-45545] - Formalin fixed, paraffin-embedded tissue after heat induced antigen retrieval. 5 ug/ml of antibody used for staining. After incubation with the ...read more
Immunohistochemistry-Paraffin: E-Selectin/CD62E Antibody [NBP1-45545] - Staining of aorta paraffin section from obese diabetic mice. Staining of Endothelial cell lair has been observed.
Immunohistochemistry-Paraffin: E-Selectin/CD62E Antibody [NBP1-45545] - Staining of aorta paraffin section from obese diabetic mice. Staining of Endothelial cell lair has been observed.
Simple Western: E-Selectin/CD62E Antibody [NBP1-45545] - Simple Western lane view shows a specific band for E Selectin/CD62E in 0.5 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

E-Selectin/CD62E Antibody Summary

Immunogen
Synthetic peptide surrounding amino acid 584 of human E-Selectin.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SELE
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 4 ug/ml
  • Simple Western
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 10 - 20 ug/ml
  • Immunoprecipitation 10 - 20 ug/ml
Theoretical MW
67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Blood Leukocyte Whole Cell Lysate
Human Blood Leukocyte Membrane Tissue Lysate (Adult Membrane Normal)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-45545 in the following applications:

Publications
Read Publications using
NBP1-45545 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2), 5 mM EDTA, 0.5% BSA, 30% Glycerol
Preservative
0.03% Proclin
Concentration
0.5 mg/ml
Purity
Affinity purified

Alternate Names for E-Selectin/CD62E Antibody

  • CD62 antigen-like family member E
  • CD62E antigen
  • CD62E
  • ELAM
  • ELAM1
  • ELAM-1
  • ELAM1E-selectin
  • endothelial adhesion molecule 1
  • Endothelial leukocyte adhesion molecule 1
  • ESEL
  • E-Selectin
  • LECAM2
  • leukocyte endothelial cell adhesion molecule 2
  • Leukocyte-endothelial cell adhesion molecule 2
  • SELE
  • selectin E

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for E-Selectin/CD62E Antibody (NBP1-45545)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-P, WB.


Filter By Application
ICC/IF
(1)
IHC
(1)
IHC-P
(3)
WB
(3)
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Filter By Species
Human
(1)
Mouse
(6)
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Showing Publications 1 - 7 of 7.
Publications using NBP1-45545 Applications Species
S. Kara, M. Akbaba, S. Kul, K. Bakır Is it possible to make early wound age estimation by immunohistochemical methods? Rom J Leg Med 2016 (IHC, Mouse) IHC Mouse
Fu X, Zhai S, Yuan J. Endothelial HuR deletion reduces the expression of proatherogenic molecules and attenuates atherosclerosis. Int. Immunopharmacol. Oct 16 2018 [PMID: 30340104] (WB, Mouse) WB Mouse
kwon TR, kim JH, Hong JY et al. Irradiation with 310 nm and 340 nm ultraviolet light-emitting-diodes can improve atopic dermatitis-like skin lesions in NC/Nga mice. Photochem. Photobiol. Sci. Jul 18 2018 [PMID: 30019049] (IHC-P, Mouse) IHC-P Mouse
Green JP, Souilhol C, Xanthis I et al. Atheroprone flow activates inflammation via endothelial ATP-dependent P2X7-p38 signalling Cardiovasc. Res. 2017 Nov 06 [PMID: 29126223] (ICC/IF, Mouse) ICC/IF Mouse
Oh CT, Kwon TR, Seok J et al. Effect of a 308-nm excimer laser on atopic dermatitis-like skin lesions in NC/Nga mice. Lasers Surg Med. 2016 Apr 13 [PMID: 27075746] (IHC-P, Mouse) IHC-P Mouse
Kwon TR, Mun SK, Oh CT et al. Therapeutic Effects of Full Spectrum Light on the Development of Atopic Dermatitis-like Lesions in NC/Nga Mice. Photochem. Photobiol. 2014 Apr 28 [PMID: 24773136] (WB, IHC-P, Mouse) WB, IHC-P Mouse
Jung Chang Hee, Lee Min Jung, Kang Yu Mi et al. Vaspin inhibits cytokine-induced nuclear factor-kappa B activation and adhesion molecule expression via AMP-activated protein kinase activation in vascular endothelial cells. Cardiovasc Diabetol. 2014 Feb 12 [PMID: 24517399] (WB, Human) WB Human

Review for E-Selectin/CD62E Antibody (NBP1-45545) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using NBP1-45545:
Filter by Applications
IF
(1)
All Applications
Filter by Species
Other
(1)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence E-Selectin/CD62E NBP1-45545
Enlarge
5
reviewed by:
Anonymous
IF Other 07/16/2015
View

Summary

ApplicationImmunofluorescence
Sample Testedcardiac tissue
SpeciesOther

Blocking

Blocking Details10% goat serum with 1% BSA

Primary Anitbody

Dilution Ratio1:33

Secondary Antibody

Secondary DescriptionAlexa Fluor 488

Details

Detection Notes-
Fixation DetailsFixed in 4.5% formaldehyde. Antigen retrieval was performed at 98C using a Kos Microwave in Tri-EDTA buffer at pH9.

Comments

CommentsPorcine cardiac tissue was stained with anti-62E antibody. In green the positive endothelial cells from veins are visible (not positive in arteries).

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for E-Selectin/CD62E Antibody (NBP1-45545) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
07/16/2015
Application: IF
Species: Other

Bioinformatics

Gene Symbol SELE
Entrez
Uniprot