E-Selectin/CD62E Antibody

Simple Western: E-Selectin/CD62E Antibody [NBP1-45545] - Simple Western lane view shows a specific band for E Selectin/CD62E in 0.5 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Western Blot: E-Selectin/CD62E Antibody [NBP1-45545] - Vaspin inhibits TNFalpha-induced expression of adhesion molecules. The effect of vaspin on TNFalpha-induced expression of E-Selectin/CD62E. The protein level and mRNA expression of each adhesion molecule was measured at 24 hr after treatment with 100 ng/mL vaspin in the presence or absence of 10 ng/mL TNFalpha. Data shown are representative Western blots (top panels) and mean values +/- SEM of three independent experiments (bottom panels). The fold change in mRNA expression compared to levels in untreated cells is shown. *p < 0.05 vs. untreated cells, and p < 0.05 vs. cells treated with TNFalpha alone. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24517399) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: E-Selectin/CD62E Antibody [NBP1-45545] - Analysis of E-Selectin in formaldehyde fixed, porcine cardiac tissue using anti-E-Selectin antibody. Image from verified customer review.

Immunohistochemistry-Paraffin: E-Selectin/CD62E Antibody [NBP1-45545] - Formalin fixed, paraffin-embedded tissue after heat induced antigen retrieval. 5 ug/ml of antibody used for staining. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

Western Blot: E-Selectin/CD62E Antibody [NBP1-45545] - Jurkat cell lysate (80 ug) loaded Blocking/Dilution buffer: 5% NFDM (1 hr at RT) / TBST E-Selectin Polyclonal Antibody used at 1:1000 dilution - O/N at 4 degrees C. Goat Anti-Rabbit IgG peroxidase conjugated used at 1:10,000 dilution - 1 hr at RT

Immunohistochemistry-Paraffin: E-Selectin/CD62E Antibody [NBP1-45545] - Staining of aorta paraffin section from obese diabetic mice. Staining of Endothelial cell lair has been observed.

Immunohistochemistry-Paraffin: E-Selectin/CD62E Antibody [NBP1-45545] - Staining of aorta paraffin section from obese diabetic mice. Staining of Endothelial cell lair has been observed.

Vaspin inhibits TNFalpha-induced expression of adhesion molecules. The effect of vaspin on TNFalpha-induced expression of ICAM-1 (A), VCAM-1 (A), E-selectin (B), and MCP-1 (B). The protein level and mRNA expression of each adhesion molecule was measured at 24 hr after treatment with 100 ng/mL vaspin in the presence or absence of 10 ng/mL TNFalpha. Data shown are representative Western blots (top panels) and mean values +/- SEM of three independent experiments (bottom panels). The fold change in mRNA expression compared to levels in untreated cells is shown. *p < 0.05 vs. untreated cells, and +p < 0.05 vs. cells treated with TNFalpha alone. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24517399), licensed under a CC-BY licence.

Product Discontinued

E-Selectin/CD62E Antibody Summary

• Description: This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab18981)
• Immunogen: Synthetic peptide surrounding amino acid 584 of human E-Selectin.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SELE
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 10-20 ug/ml
  • Immunoprecipitation 10-20 ug/ml
  • Simple Western 1:50
  • Western Blot 0.5-4 ug/ml
• Theoretical MW: 67 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Blood Leukocyte Whole Cell Lysate
  Human Blood Leukocyte Membrane Tissue Lysate (Adult Membrane Normal)

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2), 5 mM EDTA, 0.5% BSA, 30% Glycerol
• Preservative: 0.03% Proclin 300
• Concentration: 0.5 mg/ml
• Purity: Affinity purified

Alternate Names for E-Selectin/CD62E Antibody

• CD62 antigen-like family member E
• CD62E antigen
• CD62E
• ELAM
• ELAM1
• ELAM-1
• ELAM1E-selectin
• endothelial adhesion molecule 1
• Endothelial leukocyte adhesion molecule 1
• ESEL
• E-Selectin
• LECAM2
• leukocyte endothelial cell adhesion molecule 2
• Leukocyte-endothelial cell adhesion molecule 2
• SELE
• selectin E

Background

CD62E (also known as E-selectin, ELAM-1, and LECAM-1) is a 115 kD type I membrane protein and a member of the selectin family. CD62E is highly expressed on activated endothelial cells (IL-2, TNF- alpha, other cytokines can increase expression) and can also be expressed on endothelial cells in the skin, bone marrow and placenta. CD62E is involved in tethering and leucocyte rolling on activated endothelium at inflammatory sites and may also play a role in tumor metastasis and angiogenesis. CD62E binds to both Siayl Lewis X (CD15s) and PSGL-1 (CD162). The HCD62E antibody has been shown to recognize human CD62E and to be useful for flow cytometry.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for E-Selectin/CD62E Antibody (NBP1-45545)(10)

Publications using NBP1-45545

• Mastrogiacomo L, Werstuck GH Investigating the Role of Endothelial Glycogen Synthase Kinase3?/? in Atherogenesis in Low Density Lipoprotein Receptor Knockout Mice International journal of molecular sciences 2022-11-26 [PMID: 36499109] (IHC, Mouse)
  
  Details:
  Dilutions: 1:50
• Oh CT, Kwon TR, Seok J et al. Effect of a 308-nm excimer laser on atopic dermatitis-like skin lesions in NC/Nga mice. Lasers Surg Med. 2016-04-13 [PMID: 27075746] (IHC-P, Mouse)
• Fernández-Guarino M, Stewart V, Fernández-Nieto D et al. Generalized Rash in Severe Hospitalized COVID Patients: Correlation between Histological Examination, Drug Intake, Blood Interleukins Profileand Long-Term Follow-Up preprints.org 2023-09-25 (IHC-P, Human)
• Sadhukhan R, Leung J. W. C, et al. Fractionated radiation suppresses Kruppel-like factor 2 pathway to a greater extent than by single exposure to the same total dose. Sci Rep 2020-05-07 [PMID: 32382091] (Western Blot, Human)
• Fu X, Zhai S, Yuan J. Endothelial HuR deletion reduces the expression of proatherogenic molecules and attenuates atherosclerosis. Int. Immunopharmacol. 2018-10-16 [PMID: 30340104] (WB, Mouse)
• kwon TR, kim JH, Hong JY et al. Irradiation with 310 nm and 340 nm ultraviolet light-emitting-diodes can improve atopic dermatitis-like skin lesions in NC/Nga mice. Photochem. Photobiol. Sci. 2018-07-18 [PMID: 30019049] (IHC-P, Mouse)
• Green JP, Souilhol C, Xanthis I et al. Atheroprone flow activates inflammation via endothelial ATP-dependent P2X7-p38 signalling Cardiovasc. Res. 2017-11-06 [PMID: 29126223] (ICC/IF, Mouse)
• Kwon TR, Mun SK, Oh CT et al. Therapeutic Effects of Full Spectrum Light on the Development of Atopic Dermatitis-like Lesions in NC/Nga Mice. Photochem. Photobiol. 2014-04-28 [PMID: 24773136] (WB, IHC-P, Mouse)
• S. Kara, M. Akbaba, S. Kul, K. Bakır Is it possible to make early wound age estimation by immunohistochemical methods? Rom J Leg Med 2016-01-01 (IF/IHC, Mouse)
• Jung Chang Hee, Lee Min Jung, Kang Yu Mi et al. Vaspin inhibits cytokine-induced nuclear factor-kappa B activation and adhesion molecule expression via AMP-activated protein kinase activation in vascular endothelial cells. Cardiovasc Diabetol. 2014-02-12 [PMID: 24517399] (WB, Human)

Review for E-Selectin/CD62E Antibody (NBP1-45545) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Other.

reviewed by: Verified Customer IF Other 07/16/2015

Summary

• Application: Immunofluorescence
• Sample Tested: cardiac tissue
• Species: Other

Bioinformatics

• Gene Symbol: SELE
• Entrez:
  • 6401(Human)
  • 20339(Mouse)
  • 25544(Rat)
• Uniprot:
  • P16581()