E-Selectin/CD62E Antibody (RM0034-9J12)

Images

 
Immunohistochemistry-Paraffin: E-selectin Antibody (RM0034-9J12) [NB110-85473] - 4% PFA fixed and paraffin embedded mouse Lung and Heart tissue section was subjected to IHC staining of mouse E-Selectin using NB110-85473.
Immunohistochemistry-Paraffin: E-selectin Antibody (RM0034-9J12) [NB110-85473] - The Bouins solution fixed and paraffin embedded mouse kidney section from anti-GBM model was subjected to IHC with anti mouse E-Selectin ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, IHC, IHC-P
Clone
RM0034-9J12
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

E-Selectin/CD62E Antibody (RM0034-9J12) Summary

Immunogen
This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with cytokine stimulated-mouse endothelial membrane protein.
Specificity
This is specific for mouse E-Selectin
Isotype
IgG2
Clonality
Monoclonal
Host
Rat
Gene
SELE
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:400
Application Notes
Antigen retrieval: use 20ug/ml proteinase K in PBS for 15-25 mins

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
A 0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for E-Selectin/CD62E Antibody (RM0034-9J12)

  • CD62 antigen-like family member E
  • CD62E antigen
  • CD62E
  • ELAM
  • ELAM1
  • ELAM-1
  • ELAM1E-selectin
  • endothelial adhesion molecule 1
  • Endothelial leukocyte adhesion molecule 1
  • ESEL
  • E-Selectin
  • LECAM2
  • leukocyte endothelial cell adhesion molecule 2
  • Leukocyte-endothelial cell adhesion molecule 2
  • SELE
  • selectin E

Background

CD62E is also known as endothelial leukocyte adhesion molecule 1 (ELAM1) or E selectin. It is a type I membrane protein that belongs to the selectin family of surface molecules (along with CD62L and CD62P). Selectins are C type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. CD62E (E selectin) is an endothelial cell specific selectin that is expressed on cytokine induced endothelial cells only after activation with proinflammatory cytokines. In vitro experiments have shown that IL1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of CD62E in an NFkB dependent signalling cascade. CD62E has been associated with blood vessel endothelium in diverse inflammatory situations. The main ligands recognised by CD62E are oligosaccharides related to sialyl lewis x.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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FAQs for E-Selectin/CD62E Antibody (NB110-85473). (Showing 1 - 1 of 1 FAQ).

  1. This E-selectin antibody is reactive mouse, correct? I have had problems with anti-mouse E-selectin antibodies previously, as many of the commercially available antibodies have not given me the desired vessel staining in IHC. I would like to test this antibody because the image show provided shows good vessel staining.
    • Yes, this antibody is reactive with mouse. We guarantee all of our products 100% in the applications and species listed on the product datasheet. As this product is recommended for IHC-P and mouse, I think it is a good choice for you. If you are unable to get this product working in IHC-P and mouse on tissues that express the protein, then Novus would be happy to provide a full refund, or send a free of charge replacement product of equal value. View more information about the Novus 100% guarantee.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-85473AF405
Alexa Fluor 488 Labeled NB110-85473AF488
Alexa Fluor 647 Labeled NB110-85473AF647
Alexa Fluor 700 Labeled NB110-85473AF700
Allophycocyanin Labeled NB110-85473APC
DyLight 350 Labeled NB110-85473UV
DyLight 405 Labeled NB110-85473V
DyLight 488 Labeled NB110-85473G
DyLight 680 Labeled NB110-85473FR
DyLight 755 Labeled NB110-85473IR
PE Labeled NB110-85473PE
PerCP Labeled NB110-85473PCP

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Bioinformatics

Gene Symbol SELE
Entrez