E-Selectin/CD62E Antibody (RM0034-9J12) - Azide and BSA Free

Immunohistochemistry-Paraffin: E-selectin Antibody (RM0034-9J12) [NB110-85473] - 4% PFA fixed and paraffin embedded mouse Lung and Heart tissue section was subjected to IHC staining of mouse E-Selectin using NB110-85473.

Immunohistochemistry-Paraffin: E-selectin Antibody (RM0034-9J12) [NB110-85473] - The Bouins solution fixed and paraffin embedded mouse kidney section from anti-GBM model was subjected to IHC with anti mouse E-Selectin using NB110-85473.

• Reactivity: Mu
• Applications: Flow, IHC
• Clone: RM0034-9J12
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

E-Selectin/CD62E Antibody (RM0034-9J12) - Azide and BSA Free Summary

• Immunogen: Cytokine-stimulated mouse E-Selectin/CD62E
• Isotype: IgG2
• Clonality: Monoclonal
• Host: Rat
• Gene: SELE
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100 - 1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100 - 1:400
• Application Notes: Antigen retrieval: use 20ug/ml proteinase K in PBS for 15-25 mins

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein G purified
• Reconstitution Instructions: Reconstitute with sterilized PBS to a final concentration of 0.5 mg/ml.

Alternate Names for E-Selectin/CD62E Antibody (RM0034-9J12) - Azide and BSA Free

• CD62 antigen-like family member E
• CD62E antigen
• CD62E
• ELAM
• ELAM1
• ELAM-1
• ELAM1E-selectin
• endothelial adhesion molecule 1
• Endothelial leukocyte adhesion molecule 1
• ESEL
• E-Selectin
• LECAM2
• leukocyte endothelial cell adhesion molecule 2
• Leukocyte-endothelial cell adhesion molecule 2
• SELE
• selectin E

Background

CD62E is also known as endothelial leukocyte adhesion molecule 1 (ELAM1) or E selectin. It is a type I membrane protein that belongs to the selectin family of surface molecules (along with CD62L and CD62P). Selectins are C type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. CD62E (E selectin) is an endothelial cell specific selectin that is expressed on cytokine induced endothelial cells only after activation with proinflammatory cytokines. In vitro experiments have shown that IL1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of CD62E in an NFkB dependent signalling cascade. CD62E has been associated with blood vessel endothelium in diverse inflammatory situations. The main ligands recognised by CD62E are oligosaccharides related to sialyl lewis x.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for E-Selectin/CD62E Antibody (NB110-85473)(1)

Publication using NB110-85473

• Ribieras AJ, Ortiz YY, Li Y et al. E-Selectin/AAV2/2 Gene Therapy Alters Angiogenesis and Inflammatory Gene Profiles in Mouse Gangrene Model Frontiers in cardiovascular medicine 2022-06-16 [PMID: 35783833] (IHC-P, Mouse)

FAQs for E-Selectin/CD62E Antibody (NB110-85473). (Showing 1 - 1 of 1 FAQ).

1. This E-selectin antibody is reactive mouse, correct? I have had problems with anti-mouse E-selectin antibodies previously, as many of the commercially available antibodies have not given me the desired vessel staining in IHC. I would like to test this antibody because the image show provided shows good vessel staining.
   • Yes, this antibody is reactive with mouse. We guarantee all of our products 100% in the applications and species listed on the product datasheet. As this product is recommended for IHC-P and mouse, I think it is a good choice for you. If you are unable to get this product working in IHC-P and mouse on tissues that express the protein, then Novus would be happy to provide a full refund, or send a free of charge replacement product of equal value. View more information about the Novus 100% guarantee.

Bioinformatics

• Gene Symbol: SELE
• Entrez:
  • 20339(Mouse)