E-Selectin/CD62E Antibody (RM0034-9J12) [Alexa Fluor® 488]

• Reactivity: Mu
• Applications: Flow, IHC
• Clone: RM0034-9J12
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 488

E-Selectin/CD62E Antibody (RM0034-9J12) [Alexa Fluor® 488] Summary

• Immunogen: Cytokine-stimulated mouse E-Selectin/CD62E
• Isotype: IgG2
• Clonality: Monoclonal
• Host: Rat
• Gene: SELE
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for E-Selectin/CD62E Antibody (RM0034-9J12) [Alexa Fluor® 488]

• CD62 antigen-like family member E
• CD62E antigen
• CD62E
• ELAM
• ELAM1
• ELAM-1
• ELAM1E-selectin
• endothelial adhesion molecule 1
• Endothelial leukocyte adhesion molecule 1
• ESEL
• E-Selectin
• LECAM2
• leukocyte endothelial cell adhesion molecule 2
• Leukocyte-endothelial cell adhesion molecule 2
• SELE
• selectin E

Background

CD62E (also known as E-selectin, ELAM-1, and LECAM-1) is a 115 kD type I membrane protein and a member of the selectin family. CD62E is highly expressed on activated endothelial cells (IL-2, TNF- alpha, other cytokines can increase expression) and can also be expressed on endothelial cells in the skin, bone marrow and placenta. CD62E is involved in tethering and leucocyte rolling on activated endothelium at inflammatory sites and may also play a role in tumor metastasis and angiogenesis. CD62E binds to both Siayl Lewis X (CD15s) and PSGL-1 (CD162). The HCD62E antibody has been shown to recognize human CD62E and to be useful for flow cytometry.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for E-Selectin/CD62E Antibody (NB110-85473AF488)(1)

Publication using NB110-85473AF488

• Ribieras AJ, Ortiz YY, Li Y et al. E-Selectin/AAV2/2 Gene Therapy Alters Angiogenesis and Inflammatory Gene Profiles in Mouse Gangrene Model Frontiers in cardiovascular medicine 2022-06-16 [PMID: 35783833] (Immunocytochemistry/ Immunofluorescence)

Bioinformatics

• Gene Symbol: SELE