E-Selectin/CD62E Antibody (BBIG-E5) [Biotin]

E-Selectin/CD62E was detected in immersion fixed human umbilical vein endothelial cells (HUVECs) activated with TNF alpha using Mouse Anti-Human E-Selectin/CD62E Biotinylated Monoclonal Antibody (Catalog # BBA8) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, ELISA(Det), ICC/IF
• Clone: BBIG-E5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Biotin
• Concentration: LYOPH

E-Selectin/CD62E Antibody (BBIG-E5) [Biotin] Summary

• Immunogen: Activated HUVEC human umbilical vein endothelial cells
• Specificity: This antibody stains COS cells transfected with cDNA for human E-Selectin/CD62E in immunofluorescence experiments. This antibody does not stain COS cells transfected with cDNA for human ICAM-1, PECAM-1 or VCAM-1.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SELE
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-10 ug/mL
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL
• Application Notes: ELISA Capture: Human E-Selectin/CD62E Antibody (Catalog # BBA16)
  ELISA Capture: Human E-Selectin/CD62E Antibody (Catalog # BBA2)ELISA Detection: Human E-Selectin/CD62E Biotinylated Antibody (Catalog # BBA8)
  Standard: Recombinant Human E-Selectin/CD62E (Catalog # ADP1)TIMP-3 (Catalog # 973-TM)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for E-Selectin/CD62E Antibody (BBIG-E5) [Biotin]

• CD62 antigen-like family member E
• CD62E antigen
• CD62E
• ELAM
• ELAM1
• ELAM-1
• ELAM1E-selectin
• endothelial adhesion molecule 1
• Endothelial leukocyte adhesion molecule 1
• ESEL
• E-Selectin
• LECAM2
• leukocyte endothelial cell adhesion molecule 2
• Leukocyte-endothelial cell adhesion molecule 2
• SELE
• selectin E

Background

E-Selectin, also known as Endothelial Leukocyte Adhesion Molecule 1 (ELAM-1), and Leukocyte-Endothelial Cell Adhesion Molecule 2 (LECAM-2), has been designated CD62E. It is a cell-surface glycoprotein expressed by activated endothelium and it mediates the adhesion of blood neutrophils.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SELE