E-Selectin/CD62E Antibody (96409) [Biotin]

• Reactivity: Mu
• Applications: WB, ELISA(Det)
• Clone: 96409
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Biotin
• Concentration: LYOPH

E-Selectin/CD62E Antibody (96409) [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse E-Selectin
  Trp22-Pro557
  Accession # Q00690
• Specificity: Detects mouse E-Selectin/CD62E in ELISAs and Western blots. In Western blots, this antibody shows 20 - 50% cross-reactivity with recombinant human E‑Selectin and recombinant mouse (rm) L-Selectin and no cross-reactivity with rmP-Selectin or recombinant rat E-Selectin.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: SELE
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 1 ug/mL
• Application Notes: ELISA Capture: Mouse E-Selectin/CD62E Antibody (Catalog # MAB5751)
  ELISA Detection: Mouse E-Selectin/CD62E Biotinylated Antibody (Catalog # BAM5752)
  Standard: Recombinant Mouse E-Selectin/CD62E Fc Chimera (Catalog # 575-ES)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for E-Selectin/CD62E Antibody (96409) [Biotin]

• CD62 antigen-like family member E
• CD62E antigen
• CD62E
• ELAM
• ELAM1
• ELAM-1
• ELAM1E-selectin
• endothelial adhesion molecule 1
• Endothelial leukocyte adhesion molecule 1
• ESEL
• E-Selectin
• LECAM2
• leukocyte endothelial cell adhesion molecule 2
• Leukocyte-endothelial cell adhesion molecule 2
• SELE
• selectin E

Background

E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107 - 115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha , and demonstrates peak expression at 4 hours, and decay at 24 hours, in response to activation. E-Selectin ligands, expressed on neutrophils, monocytes, and a subset of memory T cells, are sialylated, fucosylated molecules which bind to the lectin domain of E-Selectin. Immunocytochemical techniques have demonstrated the expression of E-Selectin on healthy and diseased tissue. The human and mouse E-Selectin proteins share 81% amino acid similarity. E-Selectin mediates the attachment of flowing leukocytes to the blood vessel wall during inflammation by binding to E-Selectin ligands on leukocytes. These interactions are labile and permit leukocytes to roll along the vascular endothelium in the direction of blood flow. This initial interaction is followed by a stronger interaction involving ICAM-1 and VCAM-1 that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue. ELISA techniques have shown that detectable levels of soluble E-Selectin are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of E‑Selectin may be elevated in subjects with a variety of pathological conditions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for E-Selectin/CD62E Antibody (BAM5752)(1)

Publication using BAM5752

• AL Banka, MV Guevara, ER Brannon, NQ Nguyen, S Song, G Cady, DJ Pinsky, KE Uhrich, R Adili, M Holinstat, O Eniola-Ade Cargo-free particles divert neutrophil-platelet aggregates to reduce thromboinflammation Nature Communications, 2023-04-28;14(1):2462. 2023-04-28 [PMID: 37117163] (In vivo assay, Neutralization, Human, Mouse)

Bioinformatics

• Gene Symbol: SELE
• Uniprot:
  • Q00690()