Dystrophin Antibody (MANDRA1)

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Immunohistochemistry-Frozen: Dystrophin Antibody (MANDRA1) [NB120-7164] - Staining of frozen human tongue sections with 1:100 Monoclonal Anti-Dystrophin, Clone: MANDRA1 using Goat Anti-Mouse IgG, FITC-conjugate.

Product Details

Summary
Reactivity Hu, Mu, Rt, FiSpecies Glossary
Applications ELISA, ICC/IF, IHC-Fr, MiAr
Clone
MANDRA1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Dystrophin Antibody (MANDRA1) Summary

Immunogen
Fusion protein, corresponding to amino acids 3200-3684 of Human Dystrophin.
Localization
Cell Membrane
Specificity
The antibody recognizes an epitope located on the 128 amino acids at the end of the C-terminal domain of the human dystrophin molecule (amino acid residues 3558-3684).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
DMD
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry-Frozen 1:10-1:500
  • Microarray
Application Notes
ELISA: See Nyugen thi Man et al (1992) and Morris G.E et al (1993) IF: Use freshly dissected and frozen human or animal muscle tissue. Immunohistochemical staining of muscle tissue results in a clear labeling confined to the periphery (plasma membrane) of normal striated muscle fibers.
Publications
Read Publications using
NB120-7164 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
15mM Sodium Azide
Purity
Unpurified

Alternate Names for Dystrophin Antibody (MANDRA1)

  • BMDDXS272
  • CMD3B
  • DXS142
  • DXS164
  • DXS164, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, DXS272
  • DXS206
  • DXS230
  • DXS239
  • DXS268
  • DXS269
  • DXS270
  • dystrophin (muscular dystrophy, Duchenne and Becker types), includes DXS142
  • dystrophin

Background

Dystrophin is a muscle membrane protein (427 kDa) which is absent, reduced or altered as a result of mutation in Duchenne and Becker muscular dystrophies (DMD/BMD) or its homologue in the mouse.8 Severe DMD is associated with a marked dystrophin deficiency whereas patients with the milder form of BMD show less pronounced abnormalities of protein expression. Because abnormalities in the protein expression occur specifically in patients with these types of muscular dystrophy, dystrophin analysis may be used to distinguish these conditions from other neuromuscular diseases. Predictions from the sequence suggest a structural protein on the inner face of the membrane, consisting of a 25-repeat, rod-like triple-helical domain separating an N-terminal actin-binding domain from two C-terminal domains, one of which is rich in cysteine.9 The large size of dystrophin and its low abundance (<0.01% of the total muscle protein) are a hindrance to the isolation of intact, native protein for structure/function studies. Monoclonal antibodies against defined regions10 of dystrophin provide a means for studying its structure and function, interactions with other proteins and the nature of the partial gene products produced in some patients carrying deletions in the dystrophin gene. The antibodies are useful in the prenatal or post-abortion diagnosis of muscular dystrophy carriers by immunohistological analyses.11

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: ELISA, ICC/IF, IHC-Fr, MiAr

Publications for Dystrophin Antibody (NB120-7164)(3)

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics Tool for Dystrophin Antibody (NB120-7164)

Discover related pathways, diseases and genes to Dystrophin Antibody (NB120-7164). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on Dystrophin.

Could Laminin be Used to Treat Duchenne Muscular Dystrophy?
Duchenne muscular dystrophy (DMD) is a severe muscle wasting condition, causing disability and early death. There is currently no cure or adequate treatment for DMD, but pioneering research indicates that injection of a laminin protein may prevent (or...  Read full blog post.

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Bioinformatics

Gene Symbol DMD
Entrez