Dystrobrevin beta Antibody (OTI3A12) - Azide and BSA Free

Western Blot: Dystrobrevin beta Antibody (OTI3A12) - Azide and BSA Free [NBP2-70598] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Dystrobrevin beta (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Dyst

Immunocytochemistry/Immunofluorescence: Dystrobrevin beta Antibody (OTI3A12) - Azide and BSA Free [NBP2-70598] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Dystrobrevin beta.

Immunohistochemistry: Dystrobrevin beta Antibody (OTI3A12) - Azide and BSA Free [NBP2-70598] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Dystrobrevin beta mouse monoclonal antibody.

Flow Cytometry: Dystrobrevin beta Antibody (OTI3A12) - Azide and BSA Free [NBP2-70598] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Dystrobrevin beta antibody, and then analyzed by flow cytometry.

Immunohistochemistry: Dystrobrevin beta Antibody (OTI3A12) - Azide and BSA Free [NBP2-70598] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Dystrobrevin beta mouse monoclonal antibody.

Immunohistochemistry: Dystrobrevin beta Antibody (OTI3A12) - Azide and BSA Free [NBP2-70598] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Dystrobrevin beta mouse monoclonal antibody.

Immunohistochemistry: Dystrobrevin beta Antibody (OTI3A12) - Azide and BSA Free [NBP2-70598] - Staining of paraffin-embedded Human prostate tissue using anti-Dystrobrevin beta mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI3A12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Dystrobrevin beta Antibody (OTI3A12) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Dystrobrevin beta Antibody (OTI3A12) - Azide and BSA Free (NBP2-01798) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human DTNB(NP_899205) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: DTNB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 63.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Dystrobrevin beta Antibody (OTI3A12) - Azide and BSA Free

• beta-dystrobrevin
• DTN-B
• dystrobrevin beta
• dystrobrevin, beta
• MGC17163
• MGC57126

Background

Dystrobrevin beta encodes dystrobrevin beta, a component of the dystrophin-associated protein complex (DPC). The DPC consists of dystrophin and several integral and peripheral membrane proteins, including dystroglycans, sarcoglycans, syntrophins and dystrobrevin alpha and beta. The DPC localizes to the sarcolemma and its disruption is associated with various forms of muscular dystrophy. Dystrobrevin beta is thought to interact with syntrophin and the DP71 short form of dystrophin. Alternatively spliced transcript variants encoding different isoforms have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DTNB