Dynamin Antibody - Azide Free

Western Blot: Dynamin Antibody [NB100-61094] - Western Blot of rat cortex lysate using NB100-61094 at 1:1000.

Western Blot: Dynamin Antibody [NB100-61094] - Rat cortex lysate showing labeling of the ~95k Dynamin protein.

• Reactivity: Hu, Mu, Rt, Mu, Bv, Ch, Pm
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

Dynamin Antibody - Azide Free Summary

• Description: Novus Biologicals Rabbit Dynamin Antibody - Azide Free (NB100-61094) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH. Accession # Q05193
• Specificity: Specific for endogenous levels of the ~95 kDa dynamin protein.
• Predicted Species: Mouse (100%), Bovine (100%), Primate (100%), Chicken (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DNM1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 95 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

It is also anticipated that the antibody will work with human, mouse, non-human primates, cow and chicken based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for Dynamin Antibody - Azide Free

• DNM
• DNM1
• dynamin 1
• Dynamin
• dynamin-1
• EC 3.6.5.5

Background

Dynamin is a GTPase required for endocytosis of clathrin coated vesicles from the membrane. Dynamin's involvement in endocytosis was first discovered during the characterization of the temperature sensitive parylytic shibire mutation from Drosophila melanogaster. Three closely related isoforms have been identified in mammals. Dynamin1 is expressed exclusively in neurons, dynamin2 is ubiquitously expressed and dynamin3 is expressed primarily in the testes. Each of these isoforms of dynamin also has several splice variants. This suggests that each isoform/splice variant may have separate intracellular targets or function in the budding of distinct vesicle types. Common domain features of the dynamin proteins include an amino acid terminal GTPase domain, a central pleckstrin homology domain and a highly basic proline rich carboxyl terminus with several Src homology 3 (SH3) domains. These SH3 domains target the dynamin to the clathrin coated pit. Dynamin is known to interact with at least two proteins, AP2 adaptor protein complex and amphiphysin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DNM1
• Entrez:
  • 1759(Human)
  • 140694(Rat)
• Uniprot:
  • Q05193(Human)