Dynactin Subunit 2/DCTN2/DCTN-50 Antibody - BSA Free

Western Blot:[NBP3-30501]-Detection of human and mouse DCTN2 by western blot and immunoprecipitation. Samples: Whole cell lysate from Jurkat , HEK293T , HeLa and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-DCTN2 antibody used for WB at 0. 1 µg/ml and 1 µg/ml and used for IP at 6 µg/mg lysate. DCTN2 was also immunoprecipitated by rabbit anti-DCTN2 antibody , which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds and 3 seconds.

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Dynactin Subunit 2/DCTN2/DCTN-50 Antibody - BSA Free Summary

• Immunogen: between 1 and 50
• Assay Type: [Assay Type]
• Sample Volume: [Sample Volume]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DCTN2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-citrate/phosphate buffer, pH 7 to 8
• Preservative: 0.09% Sodium Azide
• Purity: Affinity purified

Alternate Names for Dynactin Subunit 2/DCTN2/DCTN-50 Antibody - BSA Free

• DCTN2
• DCTN-50
• DCTN5050 kD dynein-associated polypeptide
• dynactin 2 (p50)
• dynactin complex 50 kD subunit
• Dynactin complex 50 kDa subunit
• Dynactin Subunit 2
• dynactin subunit 2,50 kDa dynein-associated polypeptide
• Dynamitin
• p50 dynamitin
• RBP50

Background

Dynactin subunit 2 encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DCTN2