Dynactin Subunit 1/DCTN1 Antibody

Western blot shows lysates of human brain tissue, human testis tissue, mouse brain tissue, and rat testis tissue. PVDF Membrane was probed with 1 µg/mL of Human Dynactin Subunit 1/DCTN1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6657) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Dynactin Subunit 1/DCTN1 at approximately 150 and 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

DCTN1 was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain using Sheep Anti-Human/Mouse/Rat Dynactin Subunit 1/DCTN1 Antigen Affinity Purified Polyclonal Antibody (Catalog # AF6657) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Dynactin Subunit 1/DCTN1 Antibody Summary

• Immunogen: E. coli-derived recombinant human Dynactin Subunit 1/DCTN1
  Ala1145-Ser1278
  Accession # Q14203
• Specificity: Detects human Dynactin Subunit 1/DCTN1 in direct ELISAs and human, mouse and rat Dynactin Subunit 1/DCTN1 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: DCTN1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Dynactin Subunit 1/DCTN1 Antibody

• DAP-150
• DCTN1
• DP-150
• dynactin 1 (p150, Glued (Drosophila) homolog)
• Dynactin 1
• Dynactin Subunit 1
• glued homolog, Drosophila)
• HMN7B
• p135
• p150 Glued (Drosophila) homolog
• p150-glued

Background

Dynactin subunit 1 (DCTN1; also DAP-150, p150-1A and p150glued) is a 145-150 kDa member of the dynactin 150 kDa subunit family of proteins. It is a noncovalently-linked homodimer that represents the largest subunit of the neuronal dynactin complex. DCTN1 serves as a bridge that binds dynein to microtubules. This facilitates the transport of molecules along microtubules by the motor molecule dynein. Human DCTN1 is 1278 amino acids (aa) in length. It possesses an N‑terminal microtubule-association region that shows a CAP-Gly domain (aa 48-90) plus a BMBD segment (aa 115-155), and two coiled-coil domains that mediate dimerization (aa 213-547 and 943-1049). There are multiple splice variants. Two are 150 kDa in size; one is widely expressed (p150-1B) and shows a deletion of aa 132‑151, while a second is rare (p150-1AB) and shows a deletion of aa 132‑138. A third variant is 135 kDa in size and possesses a four aa substitution for aa 1-138. Other splice forms possess alternative start sites at Met19 and Met265 that may be coupled to a deletion of aa 1066‑1070 and/or a 42 aa substitution for aa 1066‑1278. Over aa 1145-1278, human DCTN1 shares 97% and 93% aa identity with mouse and rat DCTN1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DCTN1
• Uniprot:
  • Q14203(Human)