Dynactin Subunit 1/DCTN1 Antibody (1E12) - Azide and BSA Free

Sandwich ELISA: Dynactin Subunit 1/DCTN1 Antibody (1E12) [H00001639-M01] - Detection limit for recombinant GST tagged DCTN1 is 0.1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 1E12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Dynactin Subunit 1/DCTN1 Antibody (1E12) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: DCTN1 (AAH06163, 1 a.a. ~ 198 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPGPGLVKDSPLLLQQISAMRLHISQLQHENSILKGAQMKASLASLPPLHVAKLSHEGPGSELPAGALYRKTSQLLETLNQLSTHTHVVDITRTSPAAKSPSAQLMEQVAQLKSLSDTVEKLKDEVLKETVSQRPGATVPTDFATFPSSAFLRAKEEQQDDTVYMGKVTFSCAAGFGQRHRLVLTQEQLHQLHSRLIS
• Specificity: DCTN1 - dynactin 1 (p150, glued homolog, Drosophila)
• Isotype: IgG1 Lambda
• Clonality: Monoclonal
• Host: Mouse
• Gene: DCTN1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Dynactin Subunit 1/DCTN1 Antibody (1E12) - Azide and BSA Free

• DAP-150
• DCTN1
• DP-150
• dynactin 1 (p150, Glued (Drosophila) homolog)
• Dynactin 1
• Dynactin Subunit 1
• glued homolog, Drosophila)
• HMN7B
• p135
• p150 Glued (Drosophila) homolog
• p150-glued

Background

This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein. Alternative splicing of this gene results in at least 2 functionally distinct isoforms: a ubiquitously expressed one and a brain-specific one. Based on its cytogenetic location, this gene is considered as a candidate gene for limb-girdle muscular dystrophy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DCTN1
• Entrez:
  • 1639(Human)
• OMIM:
  • 105400(Human)
• Uniprot:
  • Q14203(Human)