dUTPase Antibody - Azide and BSA Free

Western Blot: dUTPase Antibody [NBP2-92887] - Analysis of extracts of various cell lines, using dUTPase at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.

Immunocytochemistry/Immunofluorescence: dUTPase Antibody [NBP2-92887] - Analysis of U-2 OS cells using dUTPase . Blue: DAPI for nuclear staining.

Immunohistochemistry-Paraffin: dUTPase Antibody [NBP2-92887] - Paraffin-embedded rat spleen using dUTPase .

Immunohistochemistry-Paraffin: dUTPase Antibody [NBP2-92887] - Paraffin-embedded human appendix using dUTPase .

Immunohistochemistry-Paraffin: dUTPase Antibody [NBP2-92887] - Paraffin-embedded human liver cancer using dUTPase .

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

dUTPase Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit dUTPase Antibody - Azide and BSA Free (NBP2-92887) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 89-252 of human DUT (NP_001020419.1). PAPGPETPAISPSKRARPAEVGGMQLRFARLSEHATAPTRGSARAAGYDLYSAYDYTIPPMEKAVVKTDIQIALPSGCYGRVAPRSGLAAKHFIDVGAGVIDEDYRGNVGVVLFNFGKEKFEVKKGDRIAQLICERIFYPEIEEVQALDDTERGSGGFGSTGKN
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DUT
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for dUTPase Antibody - Azide and BSA Free

• deoxyuridine triphosphatase
• dUTP pyrophosphatasedUTP nucleotidohydrolase
• dUTPasedeoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial
• EC 3.6.1.23
• FLJ20622

Background

dUTPase encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: DUT