dUTPase Antibody - BSA Free

Immunohistochemistry-Paraffin: dUTPase Antibody [NBP2-16241] - Immunohistochemical analysis of paraffin-embedded A549 xenograft, using antibody at 1:500 dilution.

Immunoprecipitation: dUTPase Antibody [NBP2-16241] - Immunoprecipitation of DUT protein from A431 whole cell extracts using 5 ug of DUT antibody. Western blot analysis was performed using DUT antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Western Blot: dUTPase Antibody [NBP2-16241] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with dUTPase antibody (NBP2-16241) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

dUTPase Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit dUTPase Antibody - BSA Free (NBP2-16241) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-dUTPase Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human dUTPase. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DUT
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 27 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (85%), Chicken (83%), Bovine (87%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for dUTPase Antibody - BSA Free

• deoxyuridine triphosphatase
• dUTP pyrophosphatasedUTP nucleotidohydrolase
• dUTPasedeoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial
• EC 3.6.1.23
• FLJ20622

Background

This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for dUTPase Antibody (NBP2-16241)(1)

Publication using NBP2-16241

• Wang Q, Liu X, Chen C et al. A Predictive Signature for Oxaliplatin and 5-fluorouracil Based Chemotherapy Inlocally Advanced Gastric Cancer Transl Oncol 2020-10-22 [PMID: 33091827]

Bioinformatics

• Gene Symbol: DUT