DTX1 Antibody

• Reactivity: Hu, Mu, Rt, Ca, Bv
• Applications: ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

DTX1 Antibody Summary

• Description: Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.
• Immunogen: Peptide with sequence CHHIENVLKEDAR corresponding to internal region (near N-Terminus) according to NP_004407.2.
• Epitope: internal region (near N Terminus)
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DTX1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
• Application Notes: WB: Preliminary experiments gave an approx. 48 kDa band in mouse Fetal brain lysates and in lysates of NIH3T3 after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 67.4 kDa band according to human NP_004407.2 and of 68.2 kDa band according to mouse NP_032078.2. The 48 kDa band was successfully blocked by incubation with the immunizing peptide.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DTX1 Antibody

• deltex homolog 1 (Drosophila)
• Deltex1
• DTX1
• Dx-1
• hDTX1
• hDx-1
• protein deltex-1

Background

DTX1, also known as E3 ubiquitin-protein ligase DTX1, is a 67.4 kDa, 620 amino acid protein that is involved in regulating the Notch pathway by monitoring ubiquitination and stimulating the breakdown of MEKK1. Current research is being conducted with diseases and disorders such as osteosarcoma, Noonan syndrome, and neuronitis. The protein interacts in disease and Notch signaling pathways with CRK, ITCH, UBE2D2, UBE2D3, and UBC.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DTX1 Antibody (NBP1-46126)(1)

Publication using NBP1-46126

• Gupta-Rossi N, Storck S, Griebel PJ et al. Specific over-expression of deltex and a new Kelch-like protein in human germinal center B cells. Mol Immunol 2003-03-01 [PMID: 12617994]

Bioinformatics

• Gene Symbol: DTX1
• Entrez:
  • 1840(Human)
  • 14357(Mouse)
  • 687424(Rat)
• Uniprot:
  • NP_004407.2(Human)