csl/RBPJK Antibody [Unconjugated]

CSL was detected in immersion fixed U937 human histiocytic lymphoma cell line using Goat Anti-Human CSL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4079) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

csl/RBPJK Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human CSL
  Gly208-Ser500
  Accession # Q06330
• Specificity: Detects human CSL in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RBPJ
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for csl/RBPJK Antibody [Unconjugated]

• CBF1
• CBF-1
• CSL
• H-2K binding factor-2
• IGKJRB1
• IGKJRB1RBPSUHCBF1
• IGKJRBRBP-J kappa
• immunoglobulin kappa J region recombination signal binding protein 1
• J kappa-recombination signal-binding protein
• KBF2
• RBPJ
• RBP-JK
• RBPJKMGC61669
• RBP-Jrecombining binding protein suppressor of hairless (Drosophila)
• RBPSUH
• recombination signal binding protein for immunoglobulin kappa J region
• recombining binding protein suppressor of hairless
• Renal carcinoma antigen NY-REN-30
• SUH
• suppressor of hairless homolog

Background

CSL (CBF1/RBP-Jk; Su(H)/Suppressor of Hairless; Lag-1) is a 56 kDa member of the Su(H) family of transcription factors. It is constitutively bound to DNA where it participates in gene repression. Upon ligand binding, the intracellular domain of Notch translocates to the nucleus and interacts with CSL, allowing transcription of Notch target genes. Human CSL is 500 amino acids (aa) in length. It contains three DNA binding sites (aa 58‑65; 192‑201; and 265‑297) and one IPT domain (aa 355‑445). Multiple splice variants exist that involve the first 89 N-terminal amino acids. One variant (isoform 2) is 487 aa in length and shows a 6 aa substitution for amino acids 1‑19. Over amino acids 195‑487, human CSL isoform 2 shares more than 98% aa sequence identity with mouse, rat, and canine CSL.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RBPJ
• Uniprot:
  • Q06330()