csl/RBPJK Antibody

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

csl/RBPJK Antibody Summary

Immunogen
E. coli-derived recombinant human CSL
Gly208-Ser500
Accession # Q06330
Specificity
Detects human CSL in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
RBPJ
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunocytochemistry 5-15 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for csl/RBPJK Antibody

  • CBF1
  • CBF-1
  • CSL
  • H-2K binding factor-2
  • IGKJRB1
  • IGKJRB1RBPSUHCBF1
  • IGKJRBRBP-J kappa
  • immunoglobulin kappa J region recombination signal binding protein 1
  • J kappa-recombination signal-binding protein
  • KBF2
  • RBPJ
  • RBP-JK
  • RBPJKMGC61669
  • RBP-Jrecombining binding protein suppressor of hairless (Drosophila)
  • RBPSUH
  • recombination signal binding protein for immunoglobulin kappa J region
  • recombining binding protein suppressor of hairless
  • Renal carcinoma antigen NY-REN-30
  • SUH
  • suppressor of hairless homolog

Background

CSL (CBF1/RBP-Jk; Su(H)/Suppressor of Hairless; Lag-1) is a 56 kDa member of the Su(H) family of transcription factors. It is constitutively bound to DNA where it participates in gene repression. Upon ligand binding, the intracellular domain of Notch translocates to the nucleus and interacts with CSL, allowing transcription of Notch target genes. Human CSL is 500 amino acids (aa) in length. It contains three DNA binding sites (aa 58‑65; 192‑201; and 265‑297) and one IPT domain (aa 355‑445). Multiple splice variants exist that involve the first 89 N-terminal amino acids. One variant (isoform 2) is 487 aa in length and shows a 6 aa substitution for amino acids 1‑19. Over amino acids 195‑487, human CSL isoform 2 shares more than 98% aa sequence identity with mouse, rat, and canine CSL.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol RBPJ
Entrez
Uniprot