Dtk/TYRO3 Antibody (109646) [Unconjugated]

Tyro3/Dtk was detected in immersion fixed Transfected HEK293 human embryonic kidney cells (positive) and mIMCD‑3 mouse epithelial cell line (positive) using Rat Anti-Mouse Tyro3/Dtk Monoclonal Antibody (Catalog # MAB759) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Tyro3/Dtk was detected in immersion fixed Transfected HEK293 human embryonic kidney cells (positive) and absent in wild-type HEK293 human embryonic kidney cells (negative) using Rat Anti-Mouse Tyro3/Dtk Monoclonal Antibody (Catalog # MAB759) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Expression of TAM RTKs, Gas6 and Protein S.(A) Western blot analyses of the liver lysates for the examination of TAM RTKs, Gas6 and Protein S. (B) Expression of TAM RTKs, Gas6 and Protein S in isolated liver cells: parenchymal cells (PCs), Kupffer cells(KCs) and sinusoidal endothelial cells (SECs). The primary cells were subjected to Western blotting. (C) Immunohistochemistry for the detection of TAM RTKs, Gas6 and Protein S. Arrowheads indicate PCs, and arrows indicate spindle-shaped sinusoidal cells corresponding to KCs and SECs. In negative controls (islets), sections were incubated with primary antibodies pre-incubated with an excess of blocking peptide. The livers of 15-week-old WT mice were used for the protein analyses. The images are representatives of at least three experiments. Scale bar = 20 µm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/23799121), licensed under a CC-BY license. Not internally tested by R&D Systems.

Expression of TAM RTKs, Gas6 and Protein S.(A) Western blot analyses of the liver lysates for the examination of TAM RTKs, Gas6 and Protein S. (B) Expression of TAM RTKs, Gas6 and Protein S in isolated liver cells: parenchymal cells (PCs), Kupffer cells(KCs) and sinusoidal endothelial cells (SECs). The primary cells were subjected to Western blotting. (C) Immunohistochemistry for the detection of TAM RTKs, Gas6 and Protein S. Arrowheads indicate PCs, and arrows indicate spindle-shaped sinusoidal cells corresponding to KCs and SECs. In negative controls (islets), sections were incubated with primary antibodies pre-incubated with an excess of blocking peptide. The livers of 15-week-old WT mice were used for the protein analyses. The images are representatives of at least three experiments. Scale bar = 20 µm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/23799121), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: WB, ELISA(Cap), ICC/IF
• Clone: 109646
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

Dtk/TYRO3 Antibody (109646) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Tyro3/Dtk
  Ala31-Ser418
  Accession # P55144
• Specificity: Detects mouse Tyro3/Dtk extracellular domain in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Tyro3/Dtk, recombinant mouse (rm) Mer, or rmAxl is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: Tyro3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunocytochemistry 5-25 ug/mL
  • Western Blot 1 ug/mL
• Application Notes: ELISA Detection: Mouse Dtk Biotinylated Affinity Purified PAb (Catalog number BAF759)
  Standard: Recombinant Mouse Dtk Fc Chimera (Catalog number 759-DT)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Dtk/TYRO3 Antibody (109646) [Unconjugated]

• Brt
• BYK
• Dtk
• EC 2.7.10
• EC 2.7.10.1
• FLJ16467
• Rse
• Sky
• Tif
• TYRO3 protein tyrosine kinase
• Tyro3
• Tyrosine-protein kinase byk
• Tyrosine-protein kinase DTK
• tyrosine-protein kinase receptor TYRO3
• Tyrosine-protein kinase RSE
• Tyrosine-protein kinase SKY

Background

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a new receptor tyrosine kinase subfamily. The extracellular domain of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. All three receptors bind the vitamin K-dependent protein growth-arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. The binding affinities for Gas6 is in the order of Axl > Dtk > Mer. Gas6 binding induces tyrosine phosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis. Dtk is widely expressed during embryonic development. In adults, Dtk is predominantly expressed in neurons in restricted regions of the brain.

1. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
2. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Dtk/TYRO3 Antibody (MAB759)(8)

Publications using MAB759

• Zewdie, EY;Edwards, GM;Hunter, DM;Earp, HS;Holtzhausen, A; MerTK Induces Dysfunctional Dendritic Cells by Metabolic Reprogramming Cancer immunology research 2024-07-08 [PMID: 38976507] (Mass Spectrometry, Mouse)
• J Ni, M Lin, Y Jin, J Li, Y Guo, J Zhou, G Hong, G Zhao, Z Lu Gas6 Attenuates Sepsis-Induced Tight Junction Injury and Vascular Endothelial Hyperpermeability via the Axl/NF-kappaB Signaling Pathway Front Pharmacol, 2019-06-13;10(0):662. 2019-06-13 [PMID: 31263416] (Western Blot, Mouse, Rat)
• RB Birge, C Kasikara, V Davra, D Calianese, K Geng, TE Spires, M Quigley, M Wichroski, G Sriram, L Suarez-Lop, MB Yaffe, SV Kotenko, MS De Lorenzo Pan-TAM tyrosine kinase inhibitor BMS-777607 enhances anti-PD-1 mAb efficacy in a murine model of triple-negative breast cancer Cancer Res., 2019-03-15;0(0):. 2019-03-15 [PMID: 30877108] (Flow Cytometry, Mouse)
• Fujimori T, Grabiec A, Kaur M, Bell T, Fujino N, Cook P, Svedberg F, MacDonald A, Maciewicz R, Singh D, Hussell T The Axl receptor tyrosine kinase is a discriminator of macrophage function in the inflamed lung. Mucosal Immunol, 2015-01-21;8(5):1021-30. 2015-01-21 [PMID: 25603826] (Flow Cytometry, Mouse)
• Nan Qi, Peipei Liu, Yue Zhang, Hui Wu, Yongmei Chen, Daishu Han Development of a Spontaneous Liver Disease Resembling Autoimmune Hepatitis in Mice Lacking Tyro3, Axl and Mer Receptor Tyrosine Kinases PLoS ONE 6/17/2013 [PMID: 23799121]
• Taichman , Russell, Patel , Lalit R, Bedenis , Rachel, Wang , Jingchen, Weidner , Savannah, Schumann , Taibrian, Yumoto , Kenji, Berry , Janice E, Shiozawa , Yusuke, Pienta , Kenneth GAS6 receptor status is associated with dormancy and bone metastatic tumor formation. PLoS ONE, 2013-04-24;8(4):e61873. 2013-04-24 [PMID: 23637920] (Flow Cytometry, Mouse)
• Deng T, Zhang Y, Chen Q, Yan K, Han D Toll-like receptor-mediated inhibition of Gas6 and ProS expression facilitates inflammatory cytokine production in mouse macrophages. Immunology, 2012-01-01;135(1):40-50. 2012-01-01 [PMID: 22043818] (Western Blot, Mouse)
• Shang T, Zhang X, Wang T, Sun B, Deng T, Han D Toll-like receptor-initiated testicular innate immune responses in mouse Leydig cells. Endocrinology, 2011-05-03;152(7):2827-36. 2011-05-03 [PMID: 21540291] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: Tyro3
• Uniprot:
  • P55144()