Dtk/TYRO3 Antibody (109646) [Alexa Fluor® 488]

D3 mouse embryonic stem cell line untreated (light orange filled histogram) or treated with Retinoic Acid for 48 hours (dark orange filled histogram) was stained with Rat Anti-Mouse Dtk Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB759G) or isotype control antibody (Catalog # IC005G, open histogram). View our protocol for Staining Membrane-associated Proteins.

Product Discontinued

Dtk/TYRO3 Antibody (109646) [Alexa Fluor® 488] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Dtk
  Ala31-Ser418
  Accession # P55144
• Specificity: Detects mouse Dtk extracellular domain in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Dtk, recombinant mouse (rm) Mer, or rmAxl is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: Tyro3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 5 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Dtk/TYRO3 Antibody (109646) [Alexa Fluor® 488]

• Brt
• BYK
• Dtk
• EC 2.7.10
• EC 2.7.10.1
• FLJ16467
• Rse
• Sky
• Tif
• TYRO3 protein tyrosine kinase
• Tyro3
• Tyrosine-protein kinase byk
• Tyrosine-protein kinase DTK
• tyrosine-protein kinase receptor TYRO3
• Tyrosine-protein kinase RSE
• Tyrosine-protein kinase SKY

Background

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a new receptor tyrosine kinase subfamily. The extracellular domain of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. All three receptors bind the vitamin K-dependent protein growth-arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. The binding affinities for Gas6 is in the order of Axl > Dtk > Mer. Gas6 binding induces tyrosine phosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis. Dtk is widely expressed during embryonic development. In adults, Dtk is predominantly expressed in neurons in restricted regions of the brain.

1. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
2. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Dtk/TYRO3 Antibody (FAB759G)(1)

Publication using FAB759G

• Alisha Holtzhausen, William Harris, Eric Ubil, Debra M. Hunter, Jichen Zhao, Yuewei Zhang et al. TAM Family Receptor kinase inhibition reverses MDSC-mediated suppression and augments anti-PD-1 therapy in melanoma. Cancer Immunology Research 2019-10-01 [PMID: 31451482]

Bioinformatics

• Gene Symbol: Tyro3
• Uniprot:
  • P55144()