DSU Antibody (8F9-1B2) - Azide and BSA Free

Western Blot: DSU Antibody (8F9-1B2) [H00055686-M01] - Analysis of DSU expression in transfected 293T cell line by DSU monoclonal antibody (M01), clone 8F9-1B2.Lane 1: DSU transfected lysate(25 KDa).Lane 2: Non-transfected lysate.

Western Blot: DSU Antibody (8F9-1B2) [H00055686-M01] - DSU monoclonal antibody (M01), clone 8F9-1B2 Western Blot analysis of DSU expression in MCF-7 ( Cat # L046V1 ).

Immunoprecipitation: DSU Antibody (8F9-1B2) [H00055686-M01] - Analysis of MREG transfected lysate using anti-MREG monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MREG MaxPab rabbit polyclonal antibody.

ELISA: DSU Antibody (8F9-1B2) [H00055686-M01] - Detection limit for recombinant GST tagged DSU is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clone: 8F9-1B2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

DSU Antibody (8F9-1B2) - Azide and BSA Free Summary

• Immunogen: DSU (AAH32747, 1 a.a. ~ 214 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGLRDWLRTVCCCCGCECLEERALPEKEPLVSDNNPYSSFGATLVRDDEKNLWSMPHDVSHTEADDDRTLYNLIVIRNQQAKDSEEWQKLNYDIHTLRQVRREVRNRWKCILEDLGFQKEADSLLSVTKLSTISDSKNTRKAREMLLKLAEETNIFPTSWELSERYLFVVDRLIALDAAEEFFKLARRTYPKKPGVPCLADGQKELHYLPFPSP
• Specificity: DSU - likely ortholog of mouse dilute suppressor
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MREG
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate, transfected lysate and recombinant protein for western blot. It has also been used for ELISA. Immunohistochemistry-Frozen and Immunocytochemistry/Immunofluorescence were reported in scientific literature.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 22984402).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DSU Antibody (8F9-1B2) - Azide and BSA Free

• Dilute suppressor protein homolog
• DSUFLJ10116
• FLJ10116
• melanoregulin
• MGC90296
• WDT2
• whn-dependent transcript 2

Background

DSU plays a role in the incorporation of pigments into hair. May function in membrane fusion and regulate the biogenesis of disk membranes of photoreceptor rod cells (By similarity

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DSU Antibody (H00055686-M01)(2)

Publications using H00055686-M01

• Frost LS, Lopes VS, Stefano FP et al. Loss of melanoregulin (MREG) enhances cathepsin-D secretion by the retinal pigment epithelium. Vis Neurosci 2013-04-23 [PMID: 23611523]
• Rachel RA, Nagashima K, O'Sullivan TN et al. Melanoregulin, Product of the dsu Locus, Links the BLOC-Pathway and Oa1 in Organelle Biogenesis PLoS One 2012-01-01 [PMID: 22984402] (IHC-Fr, ICC/IF, Mouse)

Bioinformatics

• Gene Symbol: MREG
• Entrez:
  • 55686(Human)
• OMIM:
  • 609207(Human)
• Uniprot:
  • AAH32747(Human)