DSCAM Antibody [Unconjugated] - Long Isoform

Western blot shows 25 ng of Recombinant Human DSCAM (Catalog # 3666-DS) and Recombinant Human DSCAM‑L1 (Catalog # 3315-DL). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human DSCAM Long Isoform Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3666) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for DSCAM at approximately 250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

DSCAM was detected in immersion fixed A172 human glioblastoma cell line using 10 µg/mL Goat Anti-Human DSCAM Long Isoform Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3666) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

DSCAM Antibody [Unconjugated] - Long Isoform Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human DSCAM long isoform
  Glu18-Met1595
  Accession # O60469
• Specificity: Detects human DSCAM in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human DSCAM-L1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DSCAM
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DSCAM Antibody [Unconjugated] - Long Isoform

• CHD2-42
• CHD2-52
• Down syndrome cell adhesion molecule
• DSCAM
• human CHD2-52 down syndrome cell adhesion molecule, 10CHD2

Background

Human DSCAM is a member of the Ig superfamily that maps to a region in chromosome 21 associated with Down Syndrome mental retardation. It encodes a 230 kDa type I membrane protein with a large extracellular domain comprising ten C2 type Ig like domains and six fibronectin type III repeats. The intracellular domain of human DSCAM does not share significant sequence homology with Drosophila DSCAM. Human DSCAM is expressed in axonal and dendritic processes of the central and peripheral nervous system. It is involved in hemophilia interactions and has been implicated in neurogenesis. By alternative splicing, a soluble form of human DSCAM that is identical to the transmembrane form through residue 1571 has been described. The extracellular domain of human and mouse DSCAM share 98% amino acid sequence homology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DSCAM Antibody (AF3666)(4)

Publications using AF3666

• N Arimura, M Okada, S Taya, KI Dewa, A Tsuzuki, H Uetake, S Miyashita, K Hashizume, K Shimaoka, S Egusa, T Nishioka, Y Yanagawa, K Yamakawa, YU Inoue, T Inoue, K Kaibuchi, M Hoshino DSCAM regulates delamination of neurons in the developing midbrain Science Advances, 2020;6(36):. 2020 [PMID: 32917586] (IHC, Mouse)
• FM Bruce, S Brown, JN Smith, PG Fuerst, L Erskine DSCAM promotes axon fasciculation and growth in the developing optic pathway Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):. 2017 [PMID: 28137836] (ICC, Mouse)
• Replacing the PDZ-interacting C-termini of DSCAM and DSCAML1 with epitope tags causes different phenotypic severity in different cell populations Elife, 2016;5(0):. 2016 [PMID: 27637097] (Western Blot, Mouse)
• Fuerst PG, Koizumi A, Masland RH, Burgess RW Neurite arborization and mosaic spacing in the mouse retina require DSCAM. Nature, 2008;451(7177):470-4. 2008 [PMID: 18216855] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: DSCAM
• Uniprot:
  • O60469()