DPP3 Antibody (OTI2D9) - Azide and BSA Free

Western Blot: DPP3 Antibody (OTI2D9) - Azide and BSA Free [NBP2-71541] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DPP3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DPP3.

Immunohistochemistry: DPP3 Antibody (OTI2D9) - Azide and BSA Free [NBP2-71541] - Staining of paraffin-embedded Human lymphoma tissue using anti-DPP3 mouse monoclonal antibody.

Flow Cytometry: DPP3 Antibody (OTI2D9) - Azide and BSA Free [NBP2-71541] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-DPP3 antibody, and then analyzed by flow cytometry.

Western Blot: DPP3 Antibody (OTI2D9) - Azide and BSA Free [NBP2-71541] - Analysis of extracts (35ug) from 9 different cell lines by using anti-DPP3 monoclonal antibody.

Immunohistochemistry: DPP3 Antibody (OTI2D9) - Azide and BSA Free [NBP2-71541] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-DPP3 mouse monoclonal antibody.

Immunohistochemistry: DPP3 Antibody (OTI2D9) - Azide and BSA Free [NBP2-71541] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DPP3 mouse monoclonal antibody.

Immunohistochemistry: DPP3 Antibody (OTI2D9) - Azide and BSA Free [NBP2-71541] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-DPP3 mouse monoclonal antibody.

Immunohistochemistry: DPP3 Antibody (OTI2D9) - Azide and BSA Free [NBP2-71541] - Staining of paraffin-embedded Human lymph node tissue using anti-DPP3 mouse monoclonal antibody.

Immunohistochemistry: DPP3 Antibody (OTI2D9) - Azide and BSA Free [NBP2-71541] - Staining of paraffin-embedded Human prostate tissue using anti-DPP3 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, IHC, CyTOF-ready
• Clone: OTI2D9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

DPP3 Antibody (OTI2D9) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse DPP3 Antibody (OTI2D9) - Azide and BSA Free (NBP2-01007) is a monoclonal antibody validated for use in IHC, WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human DPP3(NP_005691) produced in HEK293 cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: DPP3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
• Theoretical MW: 82.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for DPP3 Antibody (OTI2D9) - Azide and BSA Free

• Dipeptidyl aminopeptidase III
• Dipeptidyl arylamidase III
• dipeptidyl peptidase 3
• Dipeptidyl peptidase III
• dipeptidylpeptidase 3
• dipeptidyl-peptidase 3
• dipeptidylpeptidase III
• DPP III
• DPP3
• DPPIII
• EC 3.4.14.4
• FLJ11387
• FLJ22331

Background

DPP3 encodes a protein that is a member of the S9B family in clan SC of the serine proteases. This cytoplasmic protein binds a single zinc ion with its zinc-binding motif (HELLGH) and has post-proline dipeptidyl aminopeptidase activity, cleaving Xaa-Pro dipeptides from the N-termini of proteins. Increased activity of this protein is associated with endometrial and ovarian cancers. Alternate transcriptional splice variants have been characterized. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DPP3