Dopamine D2R/DRD2 Antibody (960710) [Unconjugated]

Dopamine D2 R/DRD2 was detected in immersion fixed paraffin-embedded sections of human brain (striatum) using Mouse Anti-Human Dopamine D2 R/DRD2 Monoclonal Antibody (Catalog # MAB9266) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell membranes and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Expression of MCT8 and OATP1C1 in D2-MSN (indirect pathway medium-sized spiny neurons) in human and macaque striatum. Representative confocal microscope compositions from double-stained sections for MCT8 (green) or OATP1C1 (green) and for the D2-MSN marker DRD2 (red) in the putamen. Merged images (right side) show the colocalization of the two signals. Coexpression of MCT8 and DRD2 is observed in human (A–C) and macaque (D–F) striatum. Coexpression of OATP1C1 and DRD2 is observed in human (G–I) and macaque (J–L) striatum. Counterstaining with DAPI (blue) shows nuclei of all cells. Note that in humans, the MCT8 signals are located mainly at the cell membrane, while in macaques, they are also located in the cytoplasm. D2-MSN: D2 receptor-expressing medium-sized spiny neurons; DRD2: Dopamine receptor type 2; Put: putamen. Scale bar = 50 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37298594), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: IHC
• Clone: 960710
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Dopamine D2R/DRD2 Antibody (960710) [Unconjugated] Summary

• Immunogen: Human DRD2 synthetic peptide
  Accession # P14416
• Specificity: Detects human Dopamine D2 R/DRD2 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Dopamine D2R/DRD2 Antibody (960710) [Unconjugated]

• D(2) dopamine receptor
• D2DR
• D2R
• Dopamine D2 R
• Dopamine D2 receptor
• Dopamine D2R
• dopamine receptor D2 isoform
• dopamine receptor D2
• DRD2
• seven transmembrane helix receptor

Background

Dopamine receptor D2 (DRD2) is localized to human striatum, motor cortex and neocortex. It is a highly conserved seven transmembrane receptor member of the G-protein coupled receptor 1 family, with three known isoforms. In the striatum, DRD2 suppresses voluntary activity in the striatopallidal pathway, and polymorphisms in this gene are associated with alcohol addiction, smoking behavior, schizophrenia, food addiction and post-traumatic stress disorder as well as myoclonus dystonia.  DRD2 has been shown to heterodimerize with DDR4 and 5-HT2A receptors, and traffics between the plasma membrane and intracellular pools and this localization can be modulated by several drugs.  DRD2 is localized to human striatum, motor cortex and neocortex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Dopamine D2R/DRD2 Antibody (MAB9266)(2)

Publications using MAB9266

• Wang, T;Wang, Y;Montero-Pedrazuela, A;Prensa, L;Guadaño-Ferraz, A;Rausell, E; Thyroid Hormone Transporters MCT8 and OATP1C1 Are Expressed in Projection Neurons and Interneurons of Basal Ganglia and Motor Thalamus in the Adult Human and Macaque Brains International journal of molecular sciences 2023-06-01 [PMID: 37298594] (IHC, Primate - Saimiri sciureus (South American Squirrel Monkey), Primate - Macaca mulatta (Rhesus Macaque), Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Human)
• AL Beyer, D Kaemmerer, J Sänger, A Lupp Co-Expression of Adaptor Protein FAM159B with Different Markers for Neuroendocrine Cells: An Immunocytochemical and Immunohistochemical Study International Journal of Molecular Sciences, 2022-11-04;23(21):. 2022-11-04 [PMID: 36362289] (ICC, Human)

Bioinformatics

• Uniprot:
  • P14416()