DOK1 [p Tyr398] Antibody

Images

 
Western Blot: DOK1 [p Tyr398] Antibody [NB100-82113] - Analysis of extracts from K562 cells untreated or treated with H2O2 using p62Dok(phospho-Tyr398) antibody
Immunohistochemistry-Paraffin: DOK1 [p Tyr398] Antibody [NB100-82113] - Immunohistochemical analysis of DOK1 (Tyr398) in paraffin-embedded human breast carcinoma tissue using NB100-82113.
Western Blot: DOK1 [p Tyr398] Antibody [NB100-82113] - Western blot analysis of extracts from K562 cells, using p62Dok (Ab-398) antibody ( Lane 1 and 2) and p62Dok (phospho-Tyr398) antibody ( Lane 3 and 4).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

DOK1 [p Tyr398] Antibody Summary

Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human p62Dok around the phosphorylation site of tyrosine 398 (E-G-YP-E-L).
Modification
p Tyr398
Specificity
p62Dok (specific to Phospho-Tyr398) detects endogenous levels of p62Dok only when phosphorylated at tyrosine 398.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DOK1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
Theoretical MW
62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
DOK1 Lysate (NBL1-09977)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, and 50% glycerol.
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DOK1 [p Tyr398] Antibody

  • docking protein 1 (downstream of tyrosine kinase 1)
  • docking protein 1
  • docking protein 1, 62kD (downstream of tyrosine kinase 1)
  • docking protein 1, 62kDa (downstream of tyrosine kinase 1)
  • Downstream of tyrosine kinase 1
  • MGC117395
  • MGC138860
  • p62(dok)
  • P62DOK
  • pp62

Background

DOK1 (P62dok, Docking protein 1) is believed to be a mainly cytoplasmic adaptor protein which down-regulates mitogen-activated protein kinase activation, inhibits cell proliferation and transformation, and promotes cell spreading and cell migration. DOK1 is a major substrate for many tyrosine kinases including c-kit, v-abl, vFPS, EGF, and PDGF. Upon phosphorylation by kinases, DOK1 forms a complex with ras GTPase-activating protein. Phosphorylation on tyrosine residues by the insulin receptor kinase results in the negative regulation of the insulin signaling pathway. DOK1 contains a putative pleckstrin homology domain at the amino terminus and ten PXXP SH3 recognition motifs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DOK1
Entrez
Uniprot