DOK1 Overexpression Lysate

Images

 
Western Blot: DOK1 Overexpression Lysate (Adult Normal) [NBL1-09977] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DOK1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

DOK1 Overexpression Lysate Summary

Description
Transient overexpression lysate of docking protein 1, 62kDa (downstream of tyrosine kinase 1) (DOK1) The lysate was created in HEK293T cells, using Plasmid ID RC217617 and based on accession number NM_001381. The protein contains a C-MYC/DDK Tag.
Gene
DOK1

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
52.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for DOK1 Overexpression Lysate

  • docking protein 1 (downstream of tyrosine kinase 1)
  • docking protein 1
  • docking protein 1, 62kD (downstream of tyrosine kinase 1)
  • docking protein 1, 62kDa (downstream of tyrosine kinase 1)
  • Downstream of tyrosine kinase 1
  • MGC117395
  • MGC138860
  • p62(dok)
  • P62DOK
  • pp62

Background

Docking protein 1 is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. Docking protein 1 contains a putative pleckstrin homology domain at the amino terminus and ten PXXP SH3 recognition motifs. Docking protein 2 binds p120 (RasGAP) from CML cells. It has been postulated to play a role in mitogenic signaling. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for DOK1 Lysate (NBL1-09977) (0)

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Bioinformatics

Gene Symbol DOK1