DNMT3A Antibody [Alexa Fluor® 700]

• Reactivity: Hu
• Applications: ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 700

DNMT3A Antibody [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant human DNMT3A
  Ala353-Lys486
  Accession # Q9Y6K1
• Specificity: Detects human DNMT3A in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human DNMT1 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DNMT3A Antibody [Alexa Fluor® 700]

• DNA (cytosine-5-)-methyltransferase 3 alpha
• DNA (cytosine-5)-methyltransferase 3A
• DNA cytosine methyltransferase 3A2
• DNA Methyltransferase 3 Alpha
• DNA methyltransferase HsaIIIA
• DNA MTase HsaIIIA
• DNMT3A
• DNMT3A2
• EC 2.1.1.37
• M.HsaIIIA
• TBRS

Background

DNMT3A (DNA [cytosine-5]-methyltransferase 3A) is a 130 kDa member of the C5-methlytransferase family of molecules. It is expressed both during development and in virtually all adult tissues except for small intestine. DNMT3A is a nuclear protein that is found associated with heterochromatin. It possesses DNA methyltransferase activity, and acts in a complex with DNMT3L in embryonic stem cells, forming a (3A)2:(3L)2 heterotetramer. It also interacts with SETDB1 to form a transcriptional repressor. Human DNMT3A is 912 amino acids (aa) in length. It contains one ProTrpTrpPro domain (aa 292-350) and one ADD-type zinc finger region (aa 494-586). There is one 100 kDa splice variant termed DNMT3a2 that contains an alternative start site at Met224, and preferentially interacts with euchromatin. Other potential splice variants show a 16 aa substitution for aa 151-912, and a 24 aa substitution for aa 1-213. Over aa 353-486, human DNMT3A shares 96% aa identity with mouse DNMT3A. 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.