Western Blot: Dnmt3a Antibody (64B1446) [Biotin] [NB100-55783] - Western blot analysis for Dnmt3a using NB100-55783 at 2 ug/ml against 10 ug of 293 cell lysate transfected with either mouse Dnmt3a (lane A) or mouse ...read more
Immunohistochemistry: Dnmt3a Antibody (64B1446) [Biotin] [NB100-55783] - Immuno-staining of Dnmt3a using NB100-55783 at 5 ug/ml dilution on cultured HeLa cells. The nucleic staining is shown in most of the cells.
Western Blot: Detects a band of approximately 120 kDa (predicted molecular weight: 102 kDa). Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Alternate Names for DNMT3A Antibody (64B1446) [Biotin]
DNA (cytosine-5-)-methyltransferase 3 alpha
DNA (cytosine-5)-methyltransferase 3A
DNA cytosine methyltransferase 3A2
DNA methyltransferase HsaIIIA
DNA MTase HsaIIIA
Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that naturally occurring mutations of Dnmt3b gene occurs in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The role of DNMT3A in development Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence. Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation. Gene silencing in DNA ... Read full blog post.
Go Ahead! Make My DNA DNA methylation plays a critical role the long-term silencing of transcription and is essential for processes such as embryonic development, germline differentiation, and tissue maturation. Dnmt3a is a member of the C5-methyltransferase family that re... Read full blog post.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
Review this Product
Be the first to review our DNMT3A Antibody (64B1446) [Biotin] and receive a gift card or discount.