DNMT3A Antibody (64B1446) [Biotin]

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Western Blot: Dnmt3a Antibody (64B1446) [Biotin] [NB100-55783] - Western blot analysis for Dnmt3a using NB100-55783 at 2 ug/ml against 10 ug of 293 cell lysate transfected with either mouse Dnmt3a (lane A) or mouse ...read more
Immunohistochemistry: Dnmt3a Antibody (64B1446) [Biotin] [NB100-55783] - Immuno-staining of Dnmt3a using NB100-55783 at 5 ug/ml dilution on cultured HeLa cells. The nucleic staining is shown in most of the cells.

Product Details

Summary
Product Discontinued
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    • Catalog Number
      NB100-55783
    • Availability
      Product Discontinued

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DNMT3A Antibody (64B1446) [Biotin] Summary

Immunogen
This DNMT3A antibody was raised against bacteria expressed recombinant mouse Dnmt3a. The epitope was found to lie near the C-terminus (a.a. 705-908), see Chen et (2002) for details.
Localization
Nuclear
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
DNMT3A
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Immunocytochemistry/Immunofluorescence 5 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Western Blot: Detects a band of approximately 120 kDa (predicted molecular weight: 102 kDa). Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Positive Control
DNMT3A Lysate (NBL1-09970)
Publications
Read Publications using
NB100-55783 in the following applications:

  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Alternate Names for DNMT3A Antibody (64B1446) [Biotin]

  • DNA (cytosine-5-)-methyltransferase 3 alpha
  • DNA (cytosine-5)-methyltransferase 3A
  • DNA cytosine methyltransferase 3A2
  • DNA methyltransferase HsaIIIA
  • DNA MTase HsaIIIA
  • DNMT3A
  • DNMT3A2
  • EC 2.1.1.37
  • M.HsaIIIA

Background

Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that naturally occurring mutations of Dnmt3b gene occurs in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DNMT3A Antibody (NB100-55783)(11)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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WB
(2)
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Mouse
(1)
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB100-55783 Applications Species
Pawlak M, Jaenisch R. De novo DNA methylation by Dnmt3a and Dnmt3b is dispensable for nuclear reprogramming of somatic cells to a pluripotent state. Genes Dev. 2011 May 15 [PMID: 21576263] (WB, Mouse)

Details:
WB: Dnmt3a (IMG-268A) & Dnmt3b (IMG-184A). Mouse induced pluripotent stem (iPSCs) cells, Fig 2.
WB Mouse
Ko CY, Hsu HC, Shen MR et al. Epigenetic silencing of CCAAT/enhancer-binding protein delta activity by YY1/polycomb group/DNA methyltransferase complex. J Biol Chem. 2008 Nov 7 [PMID: 18753137] (WB)

Details:
Antibodies cited for HeLa cells in Fig. 7A (IF), 7B (WB following a DNA Affinity Precipitation Assay of nuclear extracts), 7C and D (ChIP): 1. IMG-261A (Dnmt1) 2. IMG-268A (Dnmt3a) 3. IMG-184A (Dnmt3b).
WB
Hirasawa R, Chiba H, Kaneda M et al. Maternal and zygotic Dnmt1 are necessary and sufficient for the maintenance of DNA methylation imprints during preimplantation development. Genes Dev. 2008 Jun 15 [PMID: 18559477]

Details:
Dnmt3a (IMG-268a): IF (mouse oocytes and embryos), Fig 1. 2. Dnmt3b (IMG-184): IF (mouse oocytes and embryos), Fig 1. Note: The specificity of the antibodies was validated by IF using Dnmt3a and Dnmt3b knockout and WT mouse oocytes and embryos (Fig 1).
Linhart HG, Lin H, Yamada Y et al. Dnmt3b promotes tumorigenesis in vivo by gene-specific de novo methylation and transcriptional silencing. Genes Dev. 2007 Dec 1 [PMID: 18056424]

Details:
Products cited (mouse tumor cells): 1. IMG-184A (Dnmt3b): IHC (paraffin); Figs. 1C, 2A. Dnmt3b transgene validated in mice in Figs. 1C, 2A. 2. IMG-268A (Dnmt3a): IHC (paraffin), Wb; Figs. 1C, 2A,B. Dnmt3a transgene validated in mice in Figs. 1C, 2A,B.
Sumpter R Jr, Loo YM, Foy E et al. Regulating intracellular antiviral defense and permissiveness to hepatitis C virus RNA replication through a cellular RNA helicase, RIG-I. J Virol. 2005 Mar [PMID: 15708988]
Brenner C, Deplus R, Didelot C et al. Myc represses transcription through recruitment of DNA methyltransferase corepressor. EMBO J. 2005 Jan 26 [PMID: 15616584]
Yan Q, Cho E, Lockett S, Muegge K. Association of Lsh, a regulator of DNA methylation, with pericentromeric heterochromatin is dependent on intact heterochromatin. Mol Cell Biol. 2003 Dec [PMID: 14612388]
Fitzgerald KA, McWhirter SM, Faia KL et al. IKKepsilon and TBK1 are essential components of the IRF3 signaling pathway. Nat Immunol. 2003 May [PMID: 12692549]
Chen T, Ueda Y, Xie S, Li E. A novel Dnmt3a isoform produced from an alternative promoter localizes to euchromatin and its expression correlates with active de novo methylation. J Biol Chem. 2002 Oct 11 [PMID: 12138111]
Chariot A, Leonardi A, Muller J et al. Association of the adaptor TANK with the I kappa B kinase (IKK) regulator NEMO connects IKK complexes with IKK epsilon and TBK1 kinases. J Biol Chem. 2002 Oct 4 [PMID: 12133833]
Show All 11 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DNMT3A Antibody (NB100-55783) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NB120-13888APC
DyLight 350 Labeled NB120-13888UV
DyLight 405 Labeled NB120-13888V
DyLight 488 Labeled NB120-13888G
DyLight 550 Labeled NB120-13888R
DyLight 650 Labeled NB120-13888C
DyLight 680 Labeled NB120-13888FR
DyLight 755 Labeled NB120-13888IR
HRP Labeled NB120-13888H
PE Labeled NB120-13888PE

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Blogs on DNMT3A.

The role of DNMT3A in development
Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence.  Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation.  Gene silencing in DNA ...  Read full blog post.

Go Ahead! Make My DNA
DNA methylation plays a critical role the long-term silencing of transcription and is essential for processes such as embryonic development, germline differentiation, and tissue maturation. Dnmt3a is a member of the C5-methyltransferase family that re...  Read full blog post.

DNMT's: An Overview of 3 DNA Methyltransferases
DNA methyltransferases catalyze the transfer of the methyl group from S-andenosyl methionine (SAM) to DNA. Such methylation has wide ranging function in the cell, including organismal development and cell differentiation. In cancer, abnormal hypermeth...  Read full blog post.

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Bioinformatics

Gene Symbol DNMT3A
Entrez