Dnmt2 Antibody

Immunocytochemistry/Immunofluorescence: Dnmt2 Antibody [NBP2-98992] - Immunofluorescence staining of Dnmt2 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Dnmt2 polyclonal antibody (dilution ratio 1:5000) at 4C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to nucleus.

Product Discontinued

Dnmt2 Antibody Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human Dnmt2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRDMT1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for Dnmt2 Antibody

• DMNT2
• DNA (cytosine-5-)-methyltransferase 2
• DNA (cytosine-5)-methyltransferase-like protein 2
• DNA methyltransferase homolog HsaIIP
• DNA methyltransferase-2
• DNA MTase homolog HsaIIP
• DNMT2
• EC 2.1.1
• EC 2.1.1.29
• M.HsaIIP
• MHSAIIP
• PUMET
• RNMT1
• tRNA (cytosine-5-)-methyltransferase
• tRNA aspartic acid methyltransferase 1

Background

tRNA aspartic acid methyltransferase 1 (TRDMT1), also known as DNMT2, is a DNA methyltransferase homolog that does not methylate DNA. Instead DNMT-2 methylates cytosine-38 in the anticodon loop of aspartic acid transfer RNA. Spec ifically, DNMT-2 methylates cytosine 38 in the anticodon loop of tRNA(Asp)

DNMT2 antibodies are useful for RNA research, cell replication studies, and research on certian cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRDMT1