DNASE2B Antibody - BSA Free

Western Blot: DNASE2B Antibody [NBP2-16199] - Sample (30 ug of whole cell lysate) A: A549 12% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: DNASE2B Antibody [NBP2-16199] - Analysis of methanol-fixed A549, using antibody at 1:500 dilution.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

DNASE2B Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human DNASE2B. The exact sequence is proprietary.
• Localization: Lysosome
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DNASE2B
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (80%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for DNASE2B Antibody - BSA Free

• deoxyribonuclease II betaDNase2-like acid DNase
• DLADdeoxyribonuclease-2-beta
• DNase II beta
• DNase II-like acid DNase
• EC 3.1.22.1
• Endonuclease DLAD
• lysosomal DNase II

Background

The protein encoded by this gene shares considerable sequence similarity to, and is structurally related to DNase II. The latter is a well characterized endonuclease that catalyzes DNA hydrolysis in the absence of divalent cations at acidic pH. Unlike DNase II which is ubiquitously expressed, expression of this gene product is restricted to the salivary gland and lungs. The gene has been localized to chromosome 1p22.3 adjacent (and in opposite orientation) to the uricase pseudogene. Two transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for DNASE2B Antibody (NBP2-16199)(1)

Publication using NBP2-16199

• Sun Y, Wang X, Chen B et al. TFEB-Mediated Lysosomal Restoration Alleviates High Glucose-Induced Cataracts Via Attenuating Oxidative Stress Investigative ophthalmology & visual science 2022-06-01 [PMID: 35758908] (WB, IHC-P, Rat, Rabbit)

Bioinformatics

• Gene Symbol: DNASE2B
• Uniprot:
  • Q8WZ79(Human)