DNAJB12 Antibody

Images

 
Western Blot: DNAJB12 Antibody [NBP2-16191] - Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 10% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: DNAJB12 Antibody [NBP2-16191] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.89 mg/ml

Order Details

DNAJB12 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human DNAJB12. The exact sequence is proprietary.
Localization
Membrane
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DNAJB12
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Chicken (85%), Xenopus laevis (80%), Xenopus tropicalis (82%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.89 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DNAJB12 Antibody

  • DJ10FLJ20027
  • DKFZp586B2023
  • DnaJ (Hsp40) homolog, subfamily B, member 12
  • dnaJ homolog subfamily B member 12
  • FLJ0027

Background

DNAJB12 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus; a glycine/phenylalanine (G/F)-rich region; and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain (Ohtsuka and Hata, 2000 [PubMed 11147971]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol DNAJB12
Uniprot