DNAJB12 Antibody - BSA Free

Western Blot: DNAJB12 Antibody [NBP2-16191] - Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 10% SDS PAGE gel, diluted at 1:1000.

Immunohistochemistry-Paraffin: DNAJB12 Antibody [NBP2-16191] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:500 dilution.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

DNAJB12 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit DNAJB12 Antibody - BSA Free (NBP2-16191) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human DNAJB12. The exact sequence is proprietary.
• Localization: Membrane
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DNAJB12
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (85%), Xenopus laevis (80%), Xenopus tropicalis (82%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for DNAJB12 Antibody - BSA Free

• DJ10FLJ20027
• DKFZp586B2023
• DnaJ (Hsp40) homolog, subfamily B, member 12
• dnaJ homolog subfamily B member 12
• FLJ0027

Background

DNAJB12 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus; a glycine/phenylalanine (G/F)-rich region; and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain (Ohtsuka and Hata, 2000 [PubMed 11147971]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: DNAJB12
• Uniprot:
  • Q9NXW2(Human)