DNA polymerase sigma Antibody

Western Blot: DNA polymerase sigma Antibody [NB100-56356] - Analysis of DNA polymerase sigma in HeLa cell lysate using NB100-56356 at A) 2 ug/ml and B) 5 ug/ml.

Immunocytochemistry/Immunofluorescence: DNA polymerase sigma Antibody [NB100-56356] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-DNA polymerase sigma Antibody NB100-56356 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Cells were imaged using a 100X objective and digitally deconvolved.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

DNA polymerase sigma Antibody Summary

• Immunogen: This antibody was generated by immunizing rabbits with a synthetic peptide containing amino acids 447-462 (GTASLKAVHHMSSPAI) of human TRF4-1, GenBank no NP_008930.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TENT4A
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100 - 1:500
  • Western Blot 1:500 - 1:1000
• Application Notes: Various isoforms of TRF4-1 with different amino acid (aa) sizes, including 541, 517, 360 aa have been described.
• Control:

  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS with 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Protein G purified

Alternate Names for DNA polymerase sigma Antibody

• DNA polymerase kappa
• DNA polymerase sigma
• EC 2.7.7.7
• LAK-1TUTase 5
• PAP associated domain containing 7
• POLKTRF41
• POLSTUTASE5
• polymerase (DNA directed) sigma
• polymerase (DNA-directed) sigma
• Terminal uridylyltransferase 5
• Topoisomerase-related function protein 4-1
• TRF4-1LAK1
• TRF4PAP-associated domain-containing protein 7

Background

TRF4-1 is a nuclear protein whose expression is cell cycle-regulated at a post-transcriptional level. Two genes, TRF4-1 and TRF4-2, belonging to this family have been identified. TRF4 is also required to maintain a different aspect of higher order chromosome structure, sister chromatid cohesion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TENT4A
• Entrez:
  • 11044(Human)
• Uniprot:
  • NP_008930.1(Human)