DNA polymerase sigma Antibody

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Western Blot: DNA polymerase sigma Antibody [NB100-56356] - Analysis of DNA polymerase sigma in HeLa cell lysate using NB100-56356 at A) 2 ug/ml and B) 5 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

DNA polymerase sigma Antibody Summary

Immunogen
This antibody was generated by immunizing rabbits with a synthetic peptide containing amino acids 447-462 (GTASLKAVHHMSSPAI) of human TRF4-1, GenBank no NP_008930.1.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PAPD7
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
Application Notes
Various isoforms of TRF4-1 with different amino acid (aa) sizes, including 541, 517, 360 aa have been described. Hence, the molecular weight(s) observed by WB will depend on the isoform(s) present in the lysate.
Positive Control
HeLa Lysate (NB800-PC1)

Reactivity Notes

Cross reacts with Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Purity
Protein G purified

Alternate Names for DNA polymerase sigma Antibody

  • DNA polymerase kappa
  • DNA polymerase sigma
  • EC 2.7.7.7
  • LAK-1TUTase 5
  • PAP associated domain containing 7
  • POLKTRF41
  • POLSTUTASE5
  • polymerase (DNA directed) sigma
  • polymerase (DNA-directed) sigma
  • Terminal uridylyltransferase 5
  • Topoisomerase-related function protein 4-1
  • TRF4-1LAK1
  • TRF4PAP-associated domain-containing protein 7

Background

TRF4-1 is a nuclear protein whose expression is cell cycle-regulated at a post-transcriptional level. Two genes, TRF4-1 and TRF4-2, belonging to this family have been identified. TRF4 is also required to maintain a different aspect of higher order chromosome structure, sister chromatid cohesion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PAPD7
Entrez
Uniprot