DNA Polymerase gamma Antibody

Images

 
Western Blot: DNA Polymerase gamma Antibody [NBP1-33633] - Sample (30 ug of whole cell lysate) A: MCF-7 5% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: DNA Polymerase gamma Antibody [NBP1-33633] - analysis of methanol-fixed HeLa.
Immunohistochemistry-Paraffin: DNA Polymerase gamma Antibody [NBP1-33633] - Paraffin-embedded Skeletal Muscle, using antibody 10 ug/ml.
Western Blot: DNA Polymerase gamma Antibody [NBP1-33633] - 50 ug Rat testis lysate/extract 5% SDS-PAGE gel, antibody dilution 1:1000.
Immunoprecipitation: DNA Polymerase gamma Antibody [NBP1-33633] - Immunoprecipitation of DNA polymerase gamma protein from MCF-7 whole cell extracts using 5 ug of DNA polymerase gamma antibody.

Product Details

Summary
Reactivity Hu, Rt, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.67 mg/ml

Order Details

DNA Polymerase gamma Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human DNA polymerase gamma. The exact sequence is proprietary.
Localization
Mitochondrion
Predicted Species
Mouse (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
POLG
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
140 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.67 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DNA Polymerase gamma Antibody

  • DNA polymerase subunit gamma-1
  • EC 2.7.7.7
  • FLJ27114
  • MDP1
  • MIRAS
  • Mitochondrial DNA polymerase catalytic subunit
  • MTDPS4A
  • PolG, catalytic subunit
  • POLG1MTDPS4B
  • PolG-alpha
  • POLGAPEO
  • polymerase (DNA directed), gamma
  • SANDO
  • SCAE

Background

As the only DNA polymerase (pol) present in mitochondria, DNA polymerase gamma (POLG) is necessarily implicated in base excision repair processes to correct oxidative damage to the mitochondrial genome. Therefore, the ability of the catalytic subunit of human POLG has been tested to participate in uracil-provoked base excision repair reconstituted in vitro with purified components. Subsequent to actions of uracil-DNA glycosylase and apurinic/apyrimidinic endonuclease, human POLG is able to fill a single nucleotide gap in the presence of a 5' terminal deoxyribose phosphate (dRP) flap. The catalytic subunit of human pol gamma catalyzes release of the dRP residue from incised apurinic/apyrimidinic sites to produce a substrate for DNA ligase. Faulty replication of the human mitochondrial genome is thought to be the cause of many diseases. The low selectivity of the mitochondrial DNA polymerase is implicated as the cause of many side effects observed in the treatment of viral infections such as HIV.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol POLG
Entrez
Uniprot