Desmocollin-3 Antibody (4D2) - Azide and BSA Free

Western Blot: Desmocollin-3 Antibody (4D2) [H00001825-M01] - DSC3 monoclonal antibody (M01), clone 4D2 Western Blot analysis of DSC3 expression in A-431 ( Cat # L015V1 ).

Immunocytochemistry/Immunofluorescence: Desmocollin-3 Antibody (4D2) [H00001825-M01] - Analysis of monoclonal antibody to DSC3 on A-431 cell. Antibody concentration 10 ug/ml.

Sandwich ELISA: Desmocollin-3 Antibody (4D2) [H00001825-M01] - Detection limit for recombinant GST tagged DSC3 is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 4D2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Desmocollin-3 Antibody (4D2) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: DSC3 (NP_001932, 585 a.a. ~ 684 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. CKPKMGYTDILAVDPDEPVHGAPFYFSLPNTSPEISRLWSLTKVNDTAARLSYQKNAGFQEYTIPITVKDRAGQAATKLLRVNLCECTHPTQCRATSRST
• Specificity: DSC3 - desmocollin 3
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: DSC3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Desmocollin-3 Antibody (4D2) - Azide and BSA Free

• Cadherin family member 3
• CDHF3
• CDHF3HT-CP
• desmocollin 3
• Desmocollin3
• Desmocollin-3
• desmocollin-4
• DSC
• DSC1
• DSC2
• DSC3
• DSC4
• DSC4desmocollin-3
• HT-CP

Background

The protein encoded by this gene is a calcium-dependent glycoprotein that is a member of the desmocollin subfamily of the cadherin superfamily. These desmosomal family members, along with the desmogleins, are found primarily in epithelial cells where they constitute the adhesive proteins of the desmosome cell-cell junction and are required for cell adhesion and desmosome formation. The desmosomal family members are arranged in two clusters on chromosome 18, occupying less than 650 kb combined. Alternative splicing results in two transcript variants encoding distinct isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DSC3
• Entrez:
  • 1825(Human)
• OMIM:
  • 600271(Human)
• Uniprot:
  • NP_001932(Human)