DNA Polymerase beta Antibody (HL1525)

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Whole zebrafish extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with DNA Polymerase beta antibody [HL1525] (NBP3-48657) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used ...read more

Product Details

Summary
Reactivity Hu, ZeSpecies Glossary
Applications WB, ICC/IF
Clone
HL1525
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

DNA Polymerase beta Antibody (HL1525) Summary

Description
Novus Biologicals Rabbit DNA Polymerase beta Antibody (HL1525) (NBP3-48657) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human DNA Polymerase beta. The exact sequence is proprietary.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
POLB
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A purified

Alternate Names for DNA Polymerase beta Antibody (HL1525)

  • DNA pol beta
  • DNA polymerase beta subunit
  • DNA Polymerase beta
  • EC 2.7.7.7
  • EC 4.2.99.-
  • MGC125976
  • POLB
  • polymerase (DNA directed), beta

Background

DNA polymerase beta comprises an amino terminal 8kDa domain and a carboxy terminal 31kDa domain. DNA Polymerase beta, a DNA repair polymerase is constitutively expressed in cultured cells. DNA polymerase beta fills single nucleotide gaps in DNA produced by the base excision repair pathway of mammalian cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DNA Polymerase beta Antibody (NBP3-48657) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for DNA Polymerase beta Antibody (NBP3-48657)

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Blogs on DNA Polymerase beta.

PARP Antibody Assays aid both Apoptosis and Cancer Research
The PARP (Poly(ADP-ribose) polymerase) protein is a zinc-dependant nuclear enzyme whose main role is to detect and repair DNA single-strand breaks (SSB). However, PARP antibody research has revealed there are at least 17 PARP proteins, which also play...  Read full blog post.

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Bioinformatics

Gene Symbol POLB