![DNA Polymerase beta antibody [HL1512] detects DNA Polymerase beta protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse esophagus.DNA Polymerase beta stained by DNA Polymerase beta antibody [HL1512] (NBP3-48656) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48656_rabbit-dna-polymerase-beta-mab-hl1512-immunohistochemistry-paraffin-1411202518344920.jpg "DNA Polymerase beta antibody [HL1512] detects DNA Polymerase beta protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse esophagus.DNA Polymerase beta stained by DNA Polymerase beta antibody [HL1512] (NBP3-48656) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
| Reactivity | Hu, Mu, Rt, ZeSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | HL1512 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DNA Polymerase beta. The exact sequence is proprietary. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | POLB |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for DNA Polymerase beta Antibody (NBP3-48656)Find related products by research area.
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PARP Antibody Assays aid both Apoptosis and Cancer Research The PARP (Poly(ADP-ribose) polymerase) protein is a zinc-dependant nuclear enzyme whose main role is to detect and repair DNA single-strand breaks (SSB). However, PARP antibody research has revealed there are at least 17 PARP proteins, which also play... Read full blog post. |
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| Gene Symbol | POLB |
![Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DNA Polymerase beta antibody [HL1512] (NBP3-48656) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48656_rabbit-dna-polymerase-beta-mab-hl1512-western-blot-1411202518365915.jpg "Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DNA Polymerase beta antibody [HL1512] (NBP3-48656) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![DNA Polymerase beta antibody [HL1512] detects DNA Polymerase beta protein by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 10 umin.Green: DNA Polymerase beta stained by DNA Polymerase beta antibody [HL1512] (NBP3-48656) diluted at 1:250.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .Scale bar= 10 um.](http://images.novusbio.com/fullsize/nbp3-48656_rabbit-dna-polymerase-beta-mab-hl1512-immunocytochemistry-immunofluorescence-141120251837499.jpg "DNA Polymerase beta antibody [HL1512] detects DNA Polymerase beta protein by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 10 umin.Green: DNA Polymerase beta stained by DNA Polymerase beta antibody [HL1512] (NBP3-48656) diluted at 1:250.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .Scale bar= 10 um.")
![Wild-type (WT) and POLB knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DNA Polymerase beta antibody [HL1512] (NBP3-48656) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-48656_rabbit-dna-polymerase-beta-mab-hl1512-western-blot-1411202518345916.jpg "Wild-type (WT) and POLB knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DNA Polymerase beta antibody [HL1512] (NBP3-48656) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Whole zebrafish extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with DNA Polymerase beta antibody [HL1512] (NBP3-48656) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48656_rabbit-dna-polymerase-beta-mab-hl1512-western-blot-1411202518412921.jpg "Whole zebrafish extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with DNA Polymerase beta antibody [HL1512] (NBP3-48656) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DNA Polymerase beta antibody [HL1512] (NBP3-48656) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48656_rabbit-dna-polymerase-beta-mab-hl1512-western-blot-1411202518371523.jpg "Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DNA Polymerase beta antibody [HL1512] (NBP3-48656) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![DNA Polymerase beta antibody [HL1512] detects DNA Polymerase beta protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.DNA Polymerase beta stained by DNA Polymerase beta antibody [HL1512] (NBP3-48656) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48656_rabbit-dna-polymerase-beta-mab-hl1512-immunohistochemistry-paraffin-1411202518382811.jpg "DNA Polymerase beta antibody [HL1512] detects DNA Polymerase beta protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.DNA Polymerase beta stained by DNA Polymerase beta antibody [HL1512] (NBP3-48656) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Whole Japanese medaka extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with DNA Polymerase beta antibody [HL1512] (NBP3-48656) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48656_rabbit-dna-polymerase-beta-mab-hl1512-western-blot-141120251842510.jpg "Whole Japanese medaka extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with DNA Polymerase beta antibody [HL1512] (NBP3-48656) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")