DNA-PKcs Antibody

Western blot shows lysates of U2OS human osteosarcoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human DNA-PKcs Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3415) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for DNA-PKcs at approximately 460 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

DNA-PKcs Antibody Summary

• Immunogen: E. coli-derived recombinant human DNA-PK_CS
  Thr3844-Trp4127
  Accession # P78527
• Specificity: Detects human DNA-PK_CS in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PRKDC
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DNA-PKcs Antibody

• DNA-dependent protein kinase catalytic subunit-like
• DNA-dependent protein kinase
• DNA-PK catalytic subunit
• DNAPK
• DNAPKcs
• DNA-PKcs
• DNPK1
• EC 2.7.11.1
• HYRC
• HYRC1
• p350
• p460
• PRKDC
• XRCC7

Background

DNA-dependent protein kinase catalytic subunit (DNA-PK_CS) is a member of the PI3-kinase related kinase (PIKK) superfamily. DNA-PK_CS is a serine/threonine kinase that, along with the DNA-binding proteins Ku70/Ku80, binds to regions of DNA double-strand breaks and is involved in double-strand break repair and VDJ recombination.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DNA-PKcs Antibody (AF3415)(1)

Publication using AF3415

• Rainwater, RR;Azevedo-Pouly, AC;Waldrip, ZJ;Hicks, BH;Callais, NA;Koss, B;Storey, AJ;Burdine, L;Burdine, MS; DNA-PKcs controls the cytotoxic T cell response to cancer and transplant allograft through regulating LAT-dependent signaling Cell reports 2026-01-06 [PMID: 41505255] (Western Blot, Human)

Bioinformatics

• Gene Symbol: PRKDC
• Uniprot:
  • P78527()