DNA Ligase I Antibody

Images

 
Western Blot: DNA Ligase I Antibody [NBP1-51968] - NBP1-51968 (0.1ug/ml) staining of HeLa nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

DNA Ligase I Antibody Summary

Immunogen
Peptide with sequence C-RVREDKQPEQATTS corresponding to internal region (near C-Terminus) according to NP_000225.1.
Epitope
C-RVREDKQPEQATTS
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LIG1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 0.3 ug/ml
  • Peptide ELISA Detection limit 1:16000
Application Notes
WB: Approx. 125 kDa band observed in HeLa nuclear lysates (calculated MW of 102 kDa band according to NP_000225.1). The observed molecular weight corresponds to earlier findings with different antibodies from other sources.
Publications
Read Publication using NBP1-51968.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for DNA Ligase I Antibody

  • DNA ligase 1
  • DNA ligase I
  • EC 6.5.1.1
  • ligase I, DNA, ATP-dependent
  • MGC117397
  • MGC130025
  • Polydeoxyribonucleotide synthase [ATP] 1
  • polydeoxyribonucleotide synthase ATP 1

Background

LIG1 encodes DNA ligase I, with functions in DNA replication and the base excision repair process. Mutations in LIG1 that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-16182
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-02280
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-41190
Species: Hu, Mu, Ch
Applications: WB, B/N, ICC/IF, IP, PLA
AF3495
Species: Hu
Applications: WB, IHC
NB100-119
Species: Hu, Mu
Applications: WB, ICC/IF, IP
AF3296
Species: Hu, Mu, Rt
Applications: WB
NB500-704
Species: Hu, Rt, ChHa
Applications: WB, ICC/IF, IHC, IHC-P
NB500-106
Species: Hu, Mu, Rt, Po, Ch, Dr, Fi, Pm, Rb, Ye, Ze
Applications: WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-FrFl
NBP1-41190
Species: Hu, Mu, Ch
Applications: WB, B/N, ICC/IF, IP, PLA
NB100-116
Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, ChIP, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA
NB100-74611
Species: Hu
Applications: WB, IHC-P, IP
NBP2-27335
Species: Hu
Applications: WB, Flow, CyTOF-ready
NBP2-38600
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-48053
Species: Hu
Applications: WB, IHC, IHC-P
NB100-477
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-01020
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
AF3688
Species: Mu
Applications: WB, Flow, CyTOF-ready, ICC
NB100-150
Species: Hu, Mu
Applications: WB, ICC/IF, IP
AF6349
Species: Hu, Mu, Rt
Applications: WB
NBP1-51968
Species: Hu
Applications: WB, PEP-ELISA

Publications for DNA Ligase I Antibody (NBP1-51968)(1)

Reviews for DNA Ligase I Antibody (NBP1-51968) (0)

There are no reviews for DNA Ligase I Antibody (NBP1-51968). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for DNA Ligase I Antibody (NBP1-51968) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional DNA Ligase I Products

Bioinformatics Tool for DNA Ligase I Antibody (NBP1-51968)

Discover related pathways, diseases and genes to DNA Ligase I Antibody (NBP1-51968). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for DNA Ligase I Antibody (NBP1-51968)

Discover more about diseases related to DNA Ligase I Antibody (NBP1-51968).
 

Pathways for DNA Ligase I Antibody (NBP1-51968)

View related products by pathway.

PTMs for DNA Ligase I Antibody (NBP1-51968)

Learn more about PTMs related to DNA Ligase I Antibody (NBP1-51968).
 

Research Areas for DNA Ligase I Antibody (NBP1-51968)

Find related products by research area.

Blogs on DNA Ligase I.

Using PCNA as an Antibody Marker
PCNA antibodies are useful biomarkers in DNA repair studies. PCNA is one of several proteins essential for the completion of nucleotide excision repair, a multi-stage process involving 20 - 30 proteins, and an important factor in repairing damage and...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our DNA Ligase I Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol LIG1
Entrez
Uniprot