DNA Ligase I Antibody

Product Discontinued

DNA Ligase I Antibody Summary

• Immunogen: Peptide with sequence C-RVREDKQPEQATTS corresponding to internal region (near C-Terminus) according to NP_000225.1.
• Epitope: C-RVREDKQPEQATTS
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: LIG1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 125 kDa band observed in HeLa nuclear lysates (calculated MW of 102 kDa band according to NP_000225.1). The observed molecular weight corresponds to earlier findings with different antibodies from other sources.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DNA Ligase I Antibody

• DNA ligase 1
• DNA Ligase I
• EC 6.5.1.1
• ligase I, DNA, ATP-dependent
• MGC117397
• MGC130025
• Polydeoxyribonucleotide synthase [ATP] 1
• polydeoxyribonucleotide synthase ATP 1

Background

LIG1 encodes DNA ligase I, with functions in DNA replication and the base excision repair process. Mutations in LIG1 that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DNA Ligase I Antibody (NBP1-51968)(1)

Publication using NBP1-51968

• Ferrari G, Rossi R, Arosio D et al. Cell cycle-dependent phosphorylation of human DNA ligase I at the cyclin-dependent kinase sites. J Biol Chem 2003-09-26 [PMID: 12851383]

Bioinformatics

• Gene Symbol: LIG1
• Entrez:
  • 3978(Human)
  • 81513(Rat)
• Uniprot:
  • NP_000225.1(Human)