DNA Ligase I Antibody

Product Discontinued

DNA Ligase I Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 200 and 250 of human DNA ligase 1 using the numbering given in entry NP_000225.1 (GeneID 3978).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LIG1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:500- 1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DNA Ligase I Antibody

• DNA ligase 1
• DNA Ligase I
• EC 6.5.1.1
• ligase I, DNA, ATP-dependent
• MGC117397
• MGC130025
• Polydeoxyribonucleotide synthase [ATP] 1
• polydeoxyribonucleotide synthase ATP 1

Background

Eukaryotic DNA ligases are ATP-dependent enzymes that catalyse the joining of single and double-strand DNA breaks, which is an essential final step in DNA replication, recombination and repair. Four biochemically distinct DNA ligases, termed ligases I-IV, have been identified in mammalian cells. DNA ligase I is functionally homologous to the DNA ligase encoded by the Saccharomyces cerevisiae Cdc9 gene. The joining of Okazaki fragments during lagging strand DNA replication in mammalian cells is due to DNA ligase I.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LIG1
• Entrez:
  • 3978(Human)
• Uniprot:
  • P18858(Human)