DNA Ligase I Antibody

Images

 
Immunohistochemistry: DNA Ligase I Antibody [NB100-58815] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-DNA Ligase 1 used at a dilution of 1:1,000 ( 1ug/ml). Detection: DAB
Immunoprecipitation: DNA Ligase I Antibody [NB100-58815] - Detection of Human DNA Ligase 1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20 percent of IP loaded) from HeLa cells. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

DNA Ligase I Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 200 and 250 of human DNA ligase 1 using the numbering given in entry NP_000225.1 (GeneID 3978).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LIG1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 2-5 ug/mg
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DNA Ligase I Antibody

  • DNA ligase 1
  • DNA ligase I
  • EC 6.5.1.1
  • ligase I, DNA, ATP-dependent
  • MGC117397
  • MGC130025
  • Polydeoxyribonucleotide synthase [ATP] 1
  • polydeoxyribonucleotide synthase ATP 1

Background

Eukaryotic DNA ligases are ATP-dependent enzymes that catalyse the joining of single and double-strand DNA breaks, which is an essential final step in DNA replication, recombination and repair. Four biochemically distinct DNA ligases, termed ligases I-IV, have been identified in mammalian cells. DNA ligase I is functionally homologous to the DNA ligase encoded by the Saccharomyces cerevisiae Cdc9 gene. The joining of Okazaki fragments during lagging strand DNA replication in mammalian cells is due to DNA ligase I.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Blogs on DNA Ligase I.

Using PCNA as an Antibody Marker
PCNA antibodies are useful biomarkers in DNA repair studies. PCNA is one of several proteins essential for the completion of nucleotide excision repair, a multi-stage process involving 20 - 30 proteins, and an important factor in repairing damage and...  Read full blog post.

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Bioinformatics

Gene Symbol LIG1
Entrez
Uniprot